ISOLATION OF RAT LIVER PLASMA MEMBRANES
Open Access
- 1 December 1970
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 47 (3), 604-618
- https://doi.org/10.1083/jcb.47.3.604
Abstract
Nucleotide pyrophosphatase and phosphodiesterase I of rat liver have been found to be localized primarily in cell particulates highly enriched with respect to the most commonly accepted plasma membrane marker, 5'-nucleotidase, and therefore should themselves be assigned a plasma membrane localization. The observation that plasma membranes sediment in isotonic sucrose with both nuclear and microsomal fractions was exploited to obtain plasma membrane preparations from each fraction. Both preparations are similar in chemical and enzymic composition. Moreover, the preparative method developed in this study appears to give the best combination of yield, purity, and reproducibility available. The question of the possible identity of nucleotide pyrophosphatase and phosphodiesterase I is considered, and evidence is presented suggesting that these activities may be manifestations of the same enzyme.Keywords
This publication has 49 references indexed in Scilit:
- Ribonuclease-susceptible charged groups at the surface of ehrlich ascites tumour cellsInternational Journal of Cancer, 1969
- A critical evaluation of the contamination, by lysosomes, of preparations of outer membrane of mitochondriaBiochemical and Biophysical Research Communications, 1968
- Characteristics of rat liver phosphatidylinositol kinase and its presence in the plasma membraneBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1967
- Incorporation of phosphate into diphosphoinositide by subcellular fractions from liverBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1965
- Lipid composition of rat liver cell membranesLife Sciences, 1965
- Intracellular distribution of sialic acid and its relationship to membranesBiochimica et Biophysica Acta, 1962
- Alterations in tissue levels of uridine diphosphate glucose dehydrogenase, uridine diphosphate glucuronic acid pyrophosphatase and glucuronyl transferase induced by substances influencing the production of ascorbic acidBiochimica et Biophysica Acta, 1962
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959
- DISTRIBUTION OF ENZYMES CLEAVING PYRIDINE NUCLEOTIDES IN ANIMAL TISSUESThe Journal of cell biology, 1957
- Isolation of pure and unaltered liver nuclei morphology and biochemical compositionExperimental Cell Research, 1956