An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol
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Open Access
- 4 December 2008
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Biotechnology
- Vol. 8 (1), 1-10
- https://doi.org/10.1186/1472-6750-8-91
Abstract
No abstract availableKeywords
This publication has 28 references indexed in Scilit:
- Single-step overlap-primer-walk polymerase chain reaction for multiple mutagenesis without overlap extensionAnalytical Biochemistry, 2008
- A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis methodAnalytical Biochemistry, 2008
- Splicing together different regions of a gene by modified polymerase chain reaction-based site-directed mutagenesisAnalytical Biochemistry, 2008
- A one-tube site-directed mutagenesis method using PCR and primer extensionAnalytical Biochemistry, 2008
- Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics effortsProteins, 2007
- [4] Site-directed mutagenesisMethods in Enzymology, 1998
- Approaches to DNA Mutagenesis: An OverviewAnalytical Biochemistry, 1997
- The "megaprimer" method of site-directed mutagenesis.1990
- A simple and rapid method for the selection of oligodeoxynucleotide-directed mutantsGene, 1988
- Rapid and efficient site-specific mutagenesis without phenotypic selection.Proceedings of the National Academy of Sciences of the United States of America, 1985