Differential Proteomics of Omental and Subcutaneous Adipose Tissue Reflects Their Unalike Biochemical and Metabolic Properties
- 9 February 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 8 (4), 1682-1693
- https://doi.org/10.1021/pr800942k
Abstract
Obesity is increasing exponentially in developed countries and constitutes a public health problem by enhancing the risk for metabolic disorder and cardiovascular disease. Differences in gene expression profiles and in metabolic and biochemical properties have been well-described between omental and subcutaneous adipose tissue in humans. Because omental adipose tissue has been strongly associated with the development of insulin resistance, type 2 diabetes and cardiovascular disease, we searched for proteins differentially expressed in these two fat depots using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and mass spectrometry (MS). In this analysis, we found 43 proteins, several of which were validated by immunoblotting and immunostaining analyses. Results demonstrated tissue-specific molecular differences in the protein makeup of the two analyzed fat depots mainly related to metabolic processes such as glucose and lipid metabolism, lipid transport, protein synthesis, protein folding, response to stress and inflammation. This suggests higher metabolic activity as well as increased cell stress in the omental compared to the subcutaneous fat. These findings provide some insights into the role of omental fat in abdominal obesity-associated co-morbidities.Keywords
This publication has 65 references indexed in Scilit:
- Abdominal Visceral and Subcutaneous Adipose Tissue CompartmentsCirculation, 2007
- Evidence for a role of developmental genes in the origin of obesity and body fat distributionProceedings of the National Academy of Sciences, 2006
- A Survey of Genes Differentially Expressed in Subcutaneous and Visceral Adipose Tissue in Men*Obesity Research, 2004
- Adipose Tissue as an Endocrine OrganJournal of Clinical Endocrinology & Metabolism, 2004
- Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and womenJournal of Lipid Research, 2004
- Integrative physiology of human adipose tissueInternational Journal of Obesity, 2003
- High Expression of Complement Components in Omental Adipose Tissue in Obese MenObesity Research, 2003
- Carboxypeptidase E and thrombospondin-1 are differently expressed in subcutaneous and visceral fat of obese subjectsCellular and Molecular Life Sciences, 2002
- Glucose Uptake and Perfusion in Subcutaneous and Visceral Adipose Tissue during Insulin Stimulation in Nonobese and Obese HumansJournal of Clinical Endocrinology & Metabolism, 2002
- Insulin resistance in type 2 diabetes: role of fatty acidsDiabetes/Metabolism Research and Reviews, 2002