Cardiomyocytes Derived from Human CardiopoieticAmniotic Fluids
Open Access
- 13 August 2018
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 8 (1), 1-12
- https://doi.org/10.1038/s41598-018-30537-z
Abstract
Human amniotic fluid (hAF) cells share characteristics of both embryonic and adult stem cells. They proliferate rapidly and can differentiate into cells of all embryonic germ layers but do not form teratomas. Embryoid-bodies obtained from hAF have cardiac differentiation potential, but terminal differentiation to cardiomyocytes (CMs) has not yet been described. Our purpose was to promote cardiac differentiation in hAFcells. Cells were exposed to inducing factors for up to 15 days. Only the subset of hAF cells expressing the multipotency markers SSEA4, OCT4 and CD90 ((Cardiopoietic)AF cells) responded to the differentiation process by increasing the expression of the cardiac transcription factors Nkx2.5 and GATA4, sarcomeric proteins (cTnT, alpha-MHC, alpha-SA), Connexin43 and atrial and ventricular markers. Furthermore, differentiated cells were positive for the calcium pumps CACNA1C and SERCA2a, with approximately 30% of (Cardiopoietic)AF-derived CM-like cells responding to caffeine or adrenergic stimulation. Some spontaneous rare beating foci were also observed. In conclusion, we demonstrated that (Cardiopoietic)AF cells might differentiate toward the cardiac lineage giving rise to CM-like cells characterized by several cardiac-specific molecular, structural, and functional properties.Funding Information
- Ministero dell'Istruzione, dell'Università e della Ricerca (SIR2014-RBSI140GLQ)
This publication has 47 references indexed in Scilit:
- Valproic Acid Confers Functional Pluripotency to Human Amniotic Fluid Stem Cells in a Transgene-free ApproachMolecular Therapy, 2012
- Human ES-cell-derived cardiomyocytes electrically couple and suppress arrhythmias in injured heartsNature, 2012
- Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells to Cardiomyocytes A Methods OverviewCirculation Research, 2012
- Evaluation of Endothelial Cells Differentiated from Amniotic Fluid-Derived Stem CellsTissue Engineering, Part A, 2012
- Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signalingProceedings of the National Academy of Sciences of the United States of America, 2012
- Systematic Analysis of Gene Expression Differences between Left and Right Atria in Different Mouse Strains and in Human Atrial TissuePLOS ONE, 2011
- Embryonic stem cell bioprinting for uniform and controlled size embryoid body formationBiomicrofluidics, 2011
- Calcium Homeostasis in Human Induced Pluripotent Stem Cell-Derived CardiomyocytesStem Cell Reviews and Reports, 2011
- In vitro cardiomyogenic potential of human amniotic fluid stem cellsJournal of Tissue Engineering and Regenerative Medicine, 2011
- Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined FactorsCell, 2006