Collection, Mapping, and Annotation of Over 28,000 cDNA Clones from japonica Rice
Top Cited Papers
- 18 July 2003
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 301 (5631), 376-379
- https://doi.org/10.1126/science.1081288
Abstract
We collected and completely sequenced 28,469 full-length complementary DNA clones from Oryza sativa L. ssp. japonica cv. Nipponbare. Through homology searches of publicly available sequence data, we assigned tentative protein functions to 21,596 clones (75.86%). Mapping of the cDNA clones to genomic DNA revealed that there are 19,000 to 20,500 transcription units in the rice genome. Protein informatics analysis against the InterPro database revealed the existence of proteins presented in rice but not in Arabidopsis. Sixty-four percent of our cDNAs are homologous to Arabidopsis proteins.Keywords
This publication has 12 references indexed in Scilit:
- Sequence and analysis of rice chromosome 4Nature, 2002
- The genome sequence and structure of rice chromosome 1Nature, 2002
- Functional Annotation of a Full-Length Arabidopsis cDNA CollectionScience, 2002
- A Draft Sequence of the Rice Genome ( Oryza sativa L. ssp. indica )Science, 2002
- A draft sequence of the rice genome (Oryza sativa L. ssp japonica)Science, 2002
- The non-coding Air RNA is required for silencing autosomal imprinted genesNature, 2002
- The InterPro database, an integrated documentation resource for protein families, domains and functional sitesNucleic Acids Research, 2001
- Arabidopsis Transcription Factors: Genome-Wide Comparative Analysis Among EukaryotesScience, 2000
- Plant cis-acting regulatory DNA elements (PLACE) database: 1999Nucleic Acids Research, 1999
- Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNAProceedings of the National Academy of Sciences of the United States of America, 1998