A Technique Whose Time Has Come
- 19 April 2002
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 296 (5567), 557-559
- https://doi.org/10.1126/science.296.5567.557
Abstract
Real-time PCR is a molecular biological technique that allows the continuous monitoring of the amplification of a target DNA molecule throughout the PCR reaction. This essay gives an overview of the real-time PCR methodology, the application of this technology to the quantification levels of nucleic acids in biological samples, and the impact of this approach on genomics and medicine.Keywords
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