Pharmacokinetics and biological activity of 2,3,7,8-tetrachlorodibenzo-p-dioxin

Abstract

Concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in rat liver and adipose tissue, and hepatic ethoxyresorufin O-deethylase (EROD) activity were studied subsequent to a single subcutaneous injection of TCDD. Two types of experiments were performed to study: (a) time-dependent changes following a single injection of 300 ng TCDD/kg body wt (points 1–4), and (b) dose-dependent changes measurable after 7 days following a single injection (points 5–7). Absorption of TCDD following a single subcutaneous injection was about 90% after 3 days and 98% after 5 days. Following a single dose of 300 ng TCDD/kg body wt peak concentrations were: liver (after 3 days): 4.7±0.9 ng/g wet wt, and adipose tissue (after 7 days): 0.82±0.07 ng/g wet wt. T1/2 of TCDD in liver was 13.6 days over the total experimental period (from day 10 to 91 of the study), apparently with an initial faster phase: 11.5 days (from day 10 to 49), and a slower period at the end of the experiment: 16.9 days (from day 49 to 91); in adipose tissue the t1/2 was 24.5 days (from day 14 to 91 of the study). Maximum induction of EROD in the liver was observed (14-fold at 300 ng TCDD/kg body wt) 3–7 days following the injection; the activity was decreased to about one third of the maximum 3 weeks after the injection; increase in total cytochrome P-450 at this dose was only about 1.4-fold at the induction maximum. The ratio of the TCDD concentrations in liver and adipose tissue increased considerably between doses of 3 ng TCDD/kg body wt (ratio∶about 0.74) and 3000 ng TCDD/kg body wt (ratio∶about 7.7). The extent of EROD induction in the liver increased dose dependently. A significant effect was first observed with a dose of 3 ng TCDD/kg body wt (activity about +32% above control activity). The corresponding tissue concentration was about 10 pg TCDD/g liver wet wt. An almost perfect linear relationship exists (when using a double-log plot) between the hepatic TCDD concentration and the EROD activity for tissue concentrations ranging from 40 to 30 000 pg TCDD/g wet wt.