Desorption of Trichoderma reesei cellulase from cellulose by a range of desorbents
- 1 July 1989
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 34 (3), 291-298
- https://doi.org/10.1002/bit.260340303
Abstract
The desorption of Trichoderma reesei cellulase from Avicel by a wide range of desorbents was measured. Emphasis was placed on desorption at alkaline pH. A maximum desorption of 65–68% Avicelase activity was achieved by contact with NaOH, pH 10.0, at 40°C for 5 min in the presence of 0.005% Triton X-100 or Tween 80. The design of a suitable desorption process using these conditions is discussed. Glycerol was also effective as a desorbent either alone or in combination with alkali and detergent. However, relatively high concentrations of glycerol were needed and the maximum desorption achieved, 68%, was not significantly greater than that with only alkali and detergent.Keywords
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