The quantitative proteomes of human‐induced pluripotent stem cells and embryonic stem cells
Open Access
- 1 January 2011
- journal article
- Published by Springer Science and Business Media LLC in Molecular Systems Biology
- Vol. 7 (1), 550
- https://doi.org/10.1038/msb.2011.84
Abstract
Assessing relevant molecular differences between human‐induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) is important, given that such differences may impact their potential therapeutic use. Controversy surrounds recent gene expression studies comparing hiPSCs and hESCs. Here, we present an in‐depth quantitative mass spectrometry‐based analysis of hESCs, two different hiPSCs and their precursor fibroblast cell lines. Our comparisons confirmed the high similarity of hESCs and hiPSCS at the proteome level as 97.8% of the proteins were found unchanged. Nevertheless, a small group of 58 proteins, mainly related to metabolism, antigen processing and cell adhesion, was found significantly differentially expressed between hiPSCs and hESCs. A comparison of the regulated proteins with previously published transcriptomic studies showed a low overlap, highlighting the emerging notion that differences between both pluripotent cell lines rather reflect experimental conditions than a recurrent molecular signature.Keywords
This publication has 78 references indexed in Scilit:
- Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell LinesCell, 2011
- Pluripotency and Cellular Reprogramming: Facts, Hypotheses, Unresolved IssuesCell, 2010
- Proteomic Analysis of Sox2-Associated Proteins During Early Stages of Mouse Embryonic Stem Cell Differentiation Identifies Sox21 as a Novel Regulator of Stem Cell FateThe International Journal of Cell Cloning, 2010
- Parkinson's Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming FactorsCell, 2009
- A High-Efficiency System for the Generation and Study of Human Induced Pluripotent Stem CellsCell Stem Cell, 2008
- Disease-Specific Induced Pluripotent Stem CellsCell, 2008
- Direct Reprogramming of Terminally Differentiated Mature B Lymphocytes to PluripotencyCell, 2008
- Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined FactorsCell, 2007
- Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined FactorsCell, 2006
- Core Transcriptional Regulatory Circuitry in Human Embryonic Stem CellsCell, 2005