Impedimetric DNA Biosensor Based on a Nanoporous Alumina Membrane for the Detection of the Specific Oligonucleotide Sequence of Dengue Virus
Open Access
- 17 June 2013
- Vol. 13 (6), 7774-7785
- https://doi.org/10.3390/s130607774
Abstract
A novel and integrated membrane sensing platform for DNA detection is developed based on an anodic aluminum oxide (AAO) membrane. Platinum electrodes (~50–100 nm thick) are coated directly on both sides of the alumina membrane to eliminate the solution resistance outside the nanopores. The electrochemical impedance technique is employed to monitor the impedance changes within the nanopores upon DNA binding. Pore resistance (Rp) linearly increases in response towards the increasing concentration of the target DNA in the range of 1 × 10−12 to 1 × 10−6 M. Moreover, the biosensor selectively differentiates the complementary sequence from single base mismatched (MM-1) strands and non-complementary strands. This study reveals a simple, selective and sensitive method to fabricate a label-free DNA biosensor.Keywords
This publication has 24 references indexed in Scilit:
- Nanoporous anodic aluminum oxide for chemical sensing and biosensorsTrAC Trends in Analytical Chemistry, 2013
- Electrochemical impedance spectroscopy characterization of nanoporous alumina dengue virus biosensorBioelectrochemistry, 2012
- Nanochannels Preparation and Application in BiosensingACS Nano, 2012
- Ultrasensitive cDNA Detection of Dengue Virus RNA Using Electrochemical Nanoporous Membrane-Based BiosensorPLOS ONE, 2012
- Electrochemical nanoporous alumina membrane-based label-free DNA biosensor for the detection of Legionella spTalanta, 2012
- A Nanochannel Array-Based Electrochemical Device for Quantitative Label-free DNA AnalysisACS Nano, 2010
- The Endosymbiotic Bacterium Wolbachia Induces Resistance to Dengue Virus in Aedes aegyptiPLoS Pathogens, 2010
- A real-time PCR procedure for detection of dengue virus serotypes 1, 2, and 3, and their quantitation in clinical and laboratory samplesJournal of Virological Methods, 2010
- A novel electrochemical biosensor based on dynamic polymerase-extending hybridization for E. coli O157:H7 DNA detectionTalanta, 2009
- Hydrothermally shrunk alumina nanopores and their application to DNA sensingThe Analyst, 2006