Virus spread and initial pathological changes in the nervous system in genital herpes simplex virus type 2 infection in mice
- 1 January 1987
- journal article
- research article
- Published by Springer Science and Business Media LLC in Acta Neuropathologica
- Vol. 72 (4), 377-388
- https://doi.org/10.1007/bf00687270
Abstract
Mice were infected by the vaginal route with the MS strain of herpes simplex virus type 2 (HSV-2). Serial vaginal cultures were used to confirm infection and to select mice for this study. Two mice were killed by perfusion on days 2–6 post infection (p.i.) and lumbar and sacral cord with cauda were fixed and embedded for electron microscopy. Semithin Epon-sections were stained for viral antigen using a rabbit anti-HSV-2 antiserum and the Avidin-Biotin (ABC) method. Thin sections from antigen-positive blocks were examined by electron microscopy, and the number and types of infected cells detected by these two methods were compared. A good correlation was found between detection of infected cells by these methods. Infected cells included neurons of dorsal root ganglia and spinal cord, satellite cells of dorsal root ganglia, non-myelinating Schwann cells, astrocytes, oligodendrocytes and arachnoidal cells. Infected cells were first detected in the cauda on day 3 p.i. and in the spinal cord on day 5 p.i. The temporal and spatial distribution of infected cells was consistent with neural spread to and within the CNS. The pathological lesions showed a good correlation with the distribution and number of infected cells and are probably due to a direct virus effect. The similar sensitivity of the Epon-ABC method to electron microscopy in detecting infected cells indicates that this method may have useful applications in both experimental and diagnostic work.Keywords
This publication has 18 references indexed in Scilit:
- Intra-axonal virus in demyelinative lesions of experimental herpes simplex type 2 infectionJournal of the Neurological Sciences, 1984
- Genital Herpes in Guinea Pigs: Pathogenesis of the Primary Infection and Description of Recurrent DiseaseThe Journal of Infectious Diseases, 1982
- The demyelinating effect of corneal HSV infections in normal and nude (athymic) miceJournal of the Neurological Sciences, 1981
- Histological study of the progression of herpes simplex virus in miceArchiv für die gesamte Virusforschung, 1980
- Technical parameters of immunostaining of osmicated tissue in epoxy sections.Journal of Histochemistry & Cytochemistry, 1979
- The Interaction of Herpes Simplex Virus with Cultures of Peripheral Nervous Tissue: an Electron Microscopic StudyJournal of General Virology, 1973
- Different Patterns of Neurologic Involvement with Herpes Simplex Virus Types 1 and 2: Isolation of Herpes Simplex Virus Type 2 from the Duffy Coat of Two Adults with MeningitisThe Journal of Infectious Diseases, 1973
- Intra-axonal Location of Herpes Simplex Virus ParticlesJournal of General Virology, 1972
- The progression of herpes simplex virus to the central nervous system of the mouseEpidemiology and Infection, 1967
- THE PATHOGENESIS OF HERPES VIRUS ENCEPHALITISThe Journal of Experimental Medicine, 1964