Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array
Top Cited Papers
Open Access
- 5 December 2016
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 35 (1), 31-34
- https://doi.org/10.1038/nbt.3737
Abstract
Multiplexed genome editing is simplified by harnessing the ability of Cpf1 to process its own pre-crRNA. Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.Keywords
This publication has 14 references indexed in Scilit:
- Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNACell, 2016
- The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNANature, 2016
- Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas SystemCell, 2015
- Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing systemProceedings of the National Academy of Sciences, 2015
- In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9Nature Biotechnology, 2014
- Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vectorNucleic Acids Research, 2014
- Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector systemScientific Reports, 2014
- Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human CellsMolecular Cell, 2014
- Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editingNature Biotechnology, 2014
- Dynamics and molecular interactions of linker of nucleoskeleton and cytoskeleton (LINC) complex proteinsJournal of Cell Science, 2009