Effectiveness of an Integrated Real-Time PCR Method for Detection of the Mycobacterium tuberculosis Complex in Smear-Negative Extrapulmonary Samples in an Area of Low Tuberculosis Prevalence
- 1 February 2012
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 50 (2), 513-515
- https://doi.org/10.1128/jcm.06467-11
Abstract
Early extrapulmonary tuberculosis (EPTB) diagnosis is particularly difficult. Among 108 smear-negative extrapulmonary samples showing a positive culture for Mycobacterium tuberculosis complex (43 body fluids and 65 nonliquid specimens), 63 (58.3%) were positive with the Xpert MTB/RIF assay (GX). GX sensitivity was quite low for samples from sterile locations (especially for pleural fluids: 26.9%) but high for some nonliquid samples, like abscess aspirates (76.5%). In summary, GX may be a useful tool to be considered for EPTB diagnosis.Keywords
This publication has 17 references indexed in Scilit:
- Disseminated tuberculosis in human immunodeficiency virus infection: ineffective immunity, polyclonal disease and high mortalityThe International Journal of Tuberculosis and Lung Disease, 2011
- Comparison of Two Molecular Methods for Rapid Diagnosis of Extrapulmonary TuberculosisJournal of Clinical Microbiology, 2011
- Comparison of the Xpert MTB/RIF Test with an IS 6110 -TaqMan Real-Time PCR Assay for Direct Detection of Mycobacterium tuberculosis in Respiratory and Nonrespiratory SpecimensJournal of Clinical Microbiology, 2011
- Feasibility, diagnostic accuracy, and effectiveness of decentralised use of the Xpert MTB/RIF test for diagnosis of tuberculosis and multidrug resistance: a multicentre implementation studyThe Lancet, 2011
- Rapid Molecular Detection of Extrapulmonary Tuberculosis by the Automated GeneXpert MTB/RIF SystemJournal of Clinical Microbiology, 2011
- Nucleic acid amplification tests in the diagnosis of tuberculous pleuritis: a systematic review and meta-analysisBMC Infectious Diseases, 2004
- Evaluation of Polymerase Chain Reaction for Detection of Mycobacterium tuberculosis in Pleural FluidChest, 2001
- The Role of Clinical Suspicion in Evaluating a New Diagnostic Test for Active TuberculosisJAMA, 2000
- Useful tests on the pleural fluid in the management of patients with pleural effusionsCurrent Opinion in Pulmonary Medicine, 1999
- Tuberculous Pleural EffusionChest, 1991