The cloning expression and tissue distribution of human PP2Cβ1
- 10 July 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 431 (1), 121-124
- https://doi.org/10.1016/s0014-5793(98)00708-x
Abstract
We have cloned a novel PP2Cβ isoform from a human liver cDNA library which codes for a protein homologous to other mammalian PP2Cβs at the N-terminus but with an extended C-terminus that is unique amongst the PP2Cs. The protein expressed in E. coli is indistinguishable from human recombinant PP2Cα in its cation dependence and insensitivity to okadaic acid. Northern blot analysis of PP2Cβ along with that of PP2Cα shows that human PP2Cs are widely expressed and are most abundant in heart and skeletal muscle.Keywords
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