Severe Acute Respiratory Syndrome Coronavirus 2−Specific Antibody Responses in Coronavirus Disease Patients
Top Cited Papers
Open Access
- 1 July 2020
- journal article
- research article
- Published by Centers for Disease Control and Prevention (CDC) in Emerging Infectious Diseases
- Vol. 26 (7), 1478-1488
- https://doi.org/10.3201/eid2607.200841
Abstract
A new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has recently emerged to cause a human pandemic. Although molecular diagnostic tests were rapidly developed, serologic assays are still lacking, yet urgently needed. Validated serologic assays are needed for contact tracing, identifying the viral reservoir, and epidemiologic studies. We developed serologic assays for detection of SARS-CoV-2 neutralizing, spike protein–specific, and nucleocapsid-specific antibodies. Using serum samples from patients with PCR-confirmed SARS-CoV-2 infections, other coronaviruses, or other respiratory pathogenic infections, we validated and tested various antigens in different in-house and commercial ELISAs. We demonstrated that most PCR-confirmed SARS-CoV-2–infected persons seroconverted by 2 weeks after disease onset. We found that commercial S1 IgG or IgA ELISAs were of lower specificity, and sensitivity varied between the 2 assays; the IgA ELISA showed higher sensitivity. Overall, the validated assays described can be instrumental for detection of SARS-CoV-2–specific antibodies for diagnostic, seroepidemiologic, and vaccine evaluation studies.Keywords
This publication has 15 references indexed in Scilit:
- Comparison of Serologic Assays for Middle East Respiratory Syndrome CoronavirusEmerging Infectious Diseases, 2019
- International Biological Reference Preparations for Epidemic Infectious DiseasesEmerging Infectious Diseases, 2019
- MERS-CoV Antibody Responses 1 Year after Symptom Onset, South Korea, 2015Emerging Infectious Diseases, 2017
- Antibody Response and Disease Severity in Healthcare Worker MERS SurvivorsEmerging Infectious Diseases, 2016
- Transmission of MERS-Coronavirus in Household ContactsThe New England Journal of Medicine, 2014
- Serological assays for emerging coronaviruses: Challenges and pitfallsVirus Research, 2014
- Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMCNature, 2013
- Lack of Peripheral Memory B Cell Responses in Recovered Patients with Severe Acute Respiratory Syndrome: A Six-Year Follow-Up StudyThe Journal of Immunology, 2011
- Chronological evolution of IgM, IgA, IgG and neutralisation antibodies after infection with SARS-associated coronavirusClinical Microbiology & Infection, 2004
- Newly discovered coronavirus as the primary cause of severe acute respiratory syndromeThe Lancet, 2003