Human Malaria Parasites in Continuous Culture
- 20 August 1976
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 193 (4254), 673-675
- https://doi.org/10.1126/science.781840
Abstract
Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38 degrees C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen (1 or 5 percent). The original parasite material, derived from an infected Aotus trivirgatus monkey, was diluted more than 100 million times by the addition of human erythrocytes at 3- or 4-day intervals. The parasites continued to reproduce in their normal asexual cycle of approximately 48 hours but were no longer highly synchronous. The have remained infective to Aotus.Keywords
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