Reduction of the extent of ischemic myocardial injury by neutrophil depletion in the dog.
- 1 May 1983
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Circulation
- Vol. 67 (5), 1016-1023
- https://doi.org/10.1161/01.cir.67.5.1016
Abstract
Accumulation of polymorphonuclear neutrophils during the acute inflammatory response may exacerbate tissue injury through the release of activated oxygen products or proteolytic enzymes or both. To assess the role of neutrophils in acute myocardial infarction, circulating neutrophil levels in dogs were reduced by 77 +/- 2% (mean +/- SEM) by administering rabbit antiserum to dog neutrophils. Acute myocardial infarction was induced in open-chest anesthetized dogs by 90 minutes of left circumflex coronary artery occlusion followed by 6 hours of reperfusion. Dogs treated with neutrophil antiserum (n = 8) developed myocardial infarcts that were an average of 43% smaller than infarcts in dogs treated with nonimmune rabbit serum (n = 7) (27.0 +/- 4.5% vs 47.1% +/- 7.5% of the area at risk, p less than 0.05). In a saline-treated control group (n = 8), infarct size was 48.0 +/- 4.7% of the area at risk, a value not significantly different from that of the nonimmune serum group but significantly greater than that in the neutrophil antiserum dogs (p less than 0.05). There were no major hemodynamic differences between groups. Histopathologic examination revealed that infarcted myocardium from dogs given saline or treated with nonimmune serum had a substantial neutrophilic infiltrate, which was virtually absent in infarcted tissue from dogs treated with neutrophil antiserum. These observations suggest that neutrophil accumulation in response to myocardial ischemia may be responsible for a substantial portion of the irreversible myocardial injury resulting from temporary coronary artery occlusion.Keywords
This publication has 18 references indexed in Scilit:
- Effect of BW755C in an occlusion-reperfusion model of ischemic myocardial injuryAmerican Heart Journal, 1983
- Cardioprotective Effects of Ibuprofen in Experimental Regional and Global Myocardial IschemiaJournal of Cardiovascular Pharmacology, 1982
- Early phase acute myocardial infarct size quantification: Validation of the triphenyl tetrazolium chloride tissue enzyme staining techniqueAmerican Heart Journal, 1981
- Complement Localization and Mediation of Ischemic Injury in Baboon MyocardiumJCI Insight, 1980
- Release of Inflammatory Mediators from Stimulated NeutrophilsThe New England Journal of Medicine, 1980
- The Biology of Oxygen RadicalsScience, 1978
- Reduction by Cobra Venom Factor of Myocardial Necrosis after Coronary Artery OcclusionJCI Insight, 1978
- THE PHLOGISTIC ROLE OF C3 LEUKOTACTIC FRAGMENTS IN MYOCARDIAL INFARCTS OF RATSThe Journal of Experimental Medicine, 1971
- The speed of healing of myocardial infarction: A study of the pathologic anatomy in seventy-two casesAmerican Heart Journal, 1939