Haemolytic activity and reactive oxygen species production of four harmful algal bloom species
- 30 March 2017
- journal article
- research article
- Published by Informa UK Limited in European Journal of Phycology
- Vol. 52 (3), 311-319
- https://doi.org/10.1080/09670262.2017.1286525
Abstract
Based on haemolytic activity and reactive oxygen species (ROS) production of Chattonella marina, Chattonella antiqua, Heterocapsa circularisquama, Alexandrium tamiyavanichii and Karenia mikimotoi, the species were categorized into four types. (1) H. circularisquama: haemolytic activity was detected in both cell suspension and cell-free culture supernatant, but with greater activity in cell suspension than in the supernatant suggesting the presence of both cell surface and secreted haemolytic agents. (2) A. tamiyavanichii: equal haemolytic activities were detected in both the cell suspension and cell-free culture supernatant suggesting the presence of only secreted haemolytic agents. (3) K. mikimotoi: haemolytic activity was detected only in the cell suspension, indicating haemolytic agents occur only on the cell surface. (4) C. marina and C. antiqua: no significant haemolytic activity was detected in either cell suspension or cell-free culture supernatant, but high ROS were detected in the cell suspensions. Heterocapsa circularisquama and K. mikimotoi showed lethal effects on rotifers (Brachionus plicatilis), whereas A. tamiyavanichii, C. marina and C. antiqua had no effect. Our results suggest that H. circularisquama, K. mikimotoi and A. tamiyavanichii produce haemolytic agents with distinct characteristics, whereas C. marina and C. antiqua have an extremely potent ability to produce ROS.Keywords
Funding Information
- Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (15K07580)
- Korea Basic Science Institute (KBSI) (C36290)
This publication has 37 references indexed in Scilit:
- Comparative studies on the fish-killing activities of Chattonella marina isolated in 1985 and Chattonella antiqua isolated in 2010, and their possible toxic factorsBioscience, Biotechnology, and Biochemistry, 2016
- Taxonomic revision ofChattonella antiqua, C. marinaandC. ovata(Raphidophyceae) based on their morphological characteristics and genetic diversityPhycologia, 2009
- Experimental exposure of the blue mussel (Mytilus edulis, L.) to the toxic dinoflagellate Alexandrium fundyense: Histopathology, immune responses, and recoveryHarmful Algae, 2008
- Motility and autotoxicity inKarenia mikimotoi(Dinophyceae)Philosophical Transactions Of The Royal Society B-Biological Sciences, 2007
- High toxicity of the novel bloom-forming species Chattonella ovata (Raphidophyceae) to cultured fishHarmful Algae, 2005
- Evidence for the production of a novel proteinaceous hemolytic exotoxin by dinoflagellate Alexandrium tayloriHarmful Algae, 2004
- Oxygen Radicals are Probably Involved in the Mortality of Yellowtail by Chattonella marinaFisheries Science, 1996
- Properties of hemagglutinins newly separated from toxic phytoplanktonBiochimica et Biophysica Acta (BBA) - General Subjects, 1995
- The biology and prediction of representative red tides associated with fish kills in JapanReviews in Fisheries Science, 1994
- A quantitative fluorimetric assay for the determination of oxidant production by polymorphonuclear leukocytes: Its use in the simultaneous fluorimetric assay of cellular activation processesAnalytical Biochemistry, 1984