Ratiometric Electrochemical Sensor for Effective and Reliable Detection of Ascorbic Acid in Living Brains
- 12 August 2015
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 87 (17), 8889-8895
- https://doi.org/10.1021/acs.analchem.5b02014
Abstract
The in vivo detection of ascorbic acid (AA), one of the physiologically important cerebral neurochemicals, is critical to probe and understand brain functions. Electrochemical sensors are convenient for AA detection. However, conventional electrochemical sensors usually suffer from several challenges, such as sluggish electron transfer kinetics for AA oxidation and poor reproducibility. To address these challenges, here we report ratiometric electrochemical sensors for effective and reliable detection of AA in living brains. The sensors were constructed by immobilizing preassembled thionine/Ketjen black (KB) nanocomposites onto glassy carbon (GC) electrodes or carbon fiber microelectrodes (CFMEs). The KB in the rationally functionalized nanocomposites efficiently facilitated AA oxidation at a relatively negative potential (∼-0.14 V) without particular physical or chemical pretreatment, forming the basis of selective measurement of AA. With a well-defined and reversible pair of redox wave at -0.22 V, the assembled thionine acted as an internal reference to substantially alleviate the lab-to-lab, person-to-person, and electrode-to-electrode variations. The in vitro experiments demonstrated that the sensors exhibited extremely high reproducibility and stability toward selective measurement of AA. More, with operational simplicity and robustness in analytical performance, the designed sensors were successfully applied to in vivo effectively, selectively, and reliably monitor the dynamic change of cerebral AA associated with pathological processes (i.e., salicylate-induced tinnitus as the model) in living rats' brains. This study not only offers a new strategy for construction of ratiometric electrochemical sensors but also opens a new way for selective and reliable detection of neurochemicals for probing brain functions.Keywords
Funding Information
- Chinese Academy of Sciences (SKLEAC201501)
- Natural Science Foundation of Jiangsu Province (BK20130561)
- National Natural Science Foundation of China (21405079, 21405081)
- Ministry of Education of the People's Republic of China (20620140617, 20620140627)
- Ministry of Science and Technology of the People's Republic of China (2015CB659400)
This publication has 37 references indexed in Scilit:
- A failure in energy metabolism and antioxidant uptake precede symptoms of Huntington’s disease in miceNature Communications, 2013
- Vitamin C induces Tet-dependent DNA demethylation and a blastocyst-like state in ES cellsNature, 2013
- Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all–iPS cell mice from terminally differentiated B cellsNature Genetics, 2012
- Metabolic oxidation regulates embryonic stem cell differentiationNature Chemical Biology, 2010
- Ascorbic Acid Prevents Blood–Brain Barrier Disruption and Sensory Deficit Caused by Sustained Compression of Primary Somatosensory CortexJournal of Cerebral Blood Flow & Metabolism, 2010
- Dynamic regional changes of extracellular ascorbic acid during global cerebral ischemia: Studied with in vivo microdialysis coupled with on-line electrochemical detectionBrain Research, 2009
- Comparative study of change in extracellular ascorbic acid in different brain ischemia/reperfusion models with in vivo microdialysis combined with on-line electrochemical detectionNeurochemistry International, 2008
- Simultaneous electrochemical detection of uric acid and ascorbic acid at a poly(N,N-dimethylaniline) film-coated GC electrodeJournal of Electroanalytical Chemistry, 2004
- Ascorbate regulation and its neuroprotective role in the brainTrends in Neurosciences, 2000
- Ascorbic acid interferences in hydrogen peroxide detecting biosensors based on electrochemically immobilized enzymesAnalytical Chemistry, 1993