Solanum nigrum produces nitric oxide via nuclear factor-κB activation in mouse peritoneal macrophages
- 1 August 2005
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in European Journal of Cancer Prevention
- Vol. 14 (4), 345-350
- https://doi.org/10.1097/00008469-200508000-00006
Abstract
Nitric oxide (NO) is an antitumour molecule produced in activated macrophages and Solanum nigrum is a plant used in oriental medicine to treat tumours. In this study using mouse peritoneal macrophages, we have examined the mechanism by which Solanum nigrum regulates NO production. When Solanum nigrum was used in combination with 20 U/ml of recombinant interferon-gamma (rIFN-gamma), there was a marked cooperative induction of NO production. The increase in NO synthesis was reflected as an increased amount of inducible NO synthase (iNOS) protein. The production of NO from rIFN-gamma plus Solanum nigrum-stimulated peritoneal macrophages was decreased by treatment with N-monomethyl-L-arginine or N-tosyl-Phe chloromethyl ketone, an iNOS inhibitor. Additionally, the increased production of NO from rIFN-gamma plus Solanum nigrum-stimulated cells was almost completely inhibited by pretreatment with 100 micromol/l of pyrrolidine dithiocarbamate, an inhibitor of nuclear factor kappaB (NF-kappaB). Furthermore, Solanum nigrum increased activation of NF-kappaB. These findings suggest that Solanum nigrum increases the production of NO by rIFN-gamma-primed macrophages and NF-kappaB plays a critical role in mediating these effects.Keywords
This publication has 29 references indexed in Scilit:
- Arginine and glutamine availability and macrophage functions in the obese insulin-resistant Zucker RatJournal of Cellular Physiology, 2004
- Inhibitory action of novel aromatic diamine compound on lipopolysaccharide‐induced nuclear translocation of NF‐κB without affecting IκB degradationFEBS Letters, 2004
- Water extract isolated fromChelidonium majusenhances nitric oxide and tumour necrosis factor-α production via nuclear factor-κB activation in mouse peritoneal macrophagesJournal of Pharmacy and Pharmacology, 2004
- Inulin stimulates NO synthesis via activation of PKC-α and protein tyrosine kinase, resulting in the activation of NF-κB by IFN-γ-primed RAW 264.7 cellsThe Journal of Nutritional Biochemistry, 2003
- A pathway through interferon‐γ is the main pathway for induction of nitric oxide upon stimulation with bacterial lipopolysaccharide in mouse peritoneal cellsEuropean Journal of Biochemistry, 2003
- Human Chorionic Gonadotropin Induces Nitric Oxide Synthase mRNA in Mouse Peritoneal MacrophagesBiochemical and Biophysical Research Communications, 1996
- THE NF-κB AND IκB PROTEINS: New Discoveries and InsightsAnnual Review of Immunology, 1996
- Serine and Cysteine Proteinase Inhibitors Prevent Nitric Oxide Production by Activated Macrophages by Interfering with Transcription of the Inducible NO Synthase GeneBiochemical and Biophysical Research Communications, 1995
- Cloning and Characterization of Inducible Nitric Oxide Synthase from Mouse MacrophagesScience, 1992
- Effect of nitric oxide synthase inhibitors on hypotension in patients with septic shockThe Lancet, 1991