The Multiple Roles of Cohesin in Meiotic Chromosome Morphogenesis and Pairing
- 1 February 2009
- journal article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 20 (3), 1030-1047
- https://doi.org/10.1091/mbc.e08-06-0637
Abstract
Sister chromatid cohesion, mediated by cohesin complexes, is laid down during DNA replication and is essential for the accurate segregation of chromosomes. Previous studies indicated that, in addition to their cohesion function, cohesins are essential for completion of recombination, pairing, meiotic chromosome axis formation, and assembly of the synaptonemal complex (SC). Using mutants in the cohesin subunit Rec8, in which phosphorylated residues were mutated to alanines, we show that cohesin phosphorylation is not only important for cohesin removal, but that cohesin's meiotic prophase functions are distinct from each other. We find pairing and SC formation to be dependent on Rec8, but independent of the presence of a sister chromatid and hence sister chromatid cohesion. We identified mutations in REC8 that differentially affect Rec8's cohesion, pairing, recombination, chromosome axis and SC assembly function. These findings define Rec8 as a key determinant of meiotic chromosome morphogenesis and a central player in multiple meiotic events.This publication has 86 references indexed in Scilit:
- Meiosis I Is Established through Division-Specific Translational Control of a CyclinCell, 2008
- Kinetochore Orientation during Meiosis Is Controlled by Aurora B and the Monopolin ComplexCell, 2007
- Two Distinct Surveillance Mechanisms Monitor Meiotic Chromosome Metabolism in Budding YeastCurrent Biology, 2006
- Cyclin-Dependent Kinase Directly Regulates Initiation of Meiotic RecombinationCell, 2006
- The FK506 Binding Protein Fpr3 Counteracts Protein Phosphatase 1 to Maintain Meiotic Recombination Checkpoint ActivityCell, 2005
- Spo13 Facilitates Monopolin Recruitment to Kinetochores and Regulates Maintenance of Centromeric Cohesion during Yeast MeiosisCurrent Biology, 2004
- DNA Damage Response Pathway Uses Histone Modification to Assemble a Double-Strand Break-Specific Cohesin DomainMolecular Cell, 2004
- The Single-End InvasionCell, 2001
- Functional Genomics Identifies MonopolinCell, 2000
- Meiosis-Specific DNA Double-Strand Breaks Are Catalyzed by Spo11, a Member of a Widely Conserved Protein FamilyCell, 1997