Use of the Accessory Genome for Characterization and Typing of Acinetobacter baumannii
- 1 April 2011
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 49 (4), 1260-1266
- https://doi.org/10.1128/jcm.02335-10
Abstract
Outbreak strains of Acinetobacter baumannii are highly clonal, and cross-infection investigations can be difficult. We sought targets based on AbaR resistance islands and on other genes found in some, but not all, sequenced isolates of A. baumannii among a set of clinical isolates ( n = 70) that included multiple representatives of a number of pulsed-field gel electrophoresis (PFGE)-defined types. These included representatives that varied in their profiles at two variable-number tandem repeat (VNTR) loci, which can provide discrimination within a PFGE cluster. Detection, or not, of each element sought provided some degree of discrimination among the set, with the presence or absence of genes coding for a phage terminase (ACICU_02185), a sialic acid synthase (ACICU_00080), a polysaccharide biosynthesis protein (AB57_0094), aphA1 , bla TEM , and integron-associated orfX (Kyoto Encyclopedia of Genes and Genomes [KEGG] no. K03830) proving the most helpful in discriminating between closely related isolates in our panel. The results support VNTR data in describing distinct populations of highly similar isolates. Such analysis, in combination with other typing methods, can inform epidemiological investigations and provide additional characterization of isolates. Most genotypes carrying bla OXA-23-like were PCR positive for a yeeA-bla OXA-23 fragment found in an AbaR4-type island, suggesting that this is widespread.Keywords
This publication has 32 references indexed in Scilit:
- High prevalence of carbapenem-hydrolysing oxacillinases in epidemiologically related and unrelated Acinetobacter baumannii clinical isolates in SpainClinical Microbiology & Infection, 2007
- Molecular Epidemiology of Imipenem-Resistant Acinetobacter haemolyticus and Acinetobacter baumannii Isolates Carrying Plasmid-Mediated OXA-40 from a Portuguese HospitalAntimicrobial Agents and Chemotherapy, 2007
- Use of sequence-based typing and multiplex PCR to identify clonal lineages of outbreak strains of Acinetobacter baumanniiClinical Microbiology & Infection, 2007
- Horizontal Gene Transfer in a Polyclonal Outbreak of Carbapenem-ResistantAcinetobacter baumanniiJournal of Clinical Microbiology, 2007
- Identification of widespread, closely related Acinetobacter baumannii isolates in Portugal as a subgroup of European clone IIClinical Microbiology & Infection, 2007
- Occurrence of Carbapenem-Resistant Acinetobacter baumannii Clones at Multiple Hospitals in London and Southeast EnglandJournal of Clinical Microbiology, 2006
- Identification of Acinetobacter baumannii by Detection of the bla OXA-51-like Carbapenemase Gene Intrinsic to This SpeciesJournal of Clinical Microbiology, 2006
- Comparison of Acinetobacter baumannii Isolates from the United Kingdom and the United States That Were Associated with Repatriated Casualties of the Iraq ConflictJournal of Clinical Microbiology, 2006
- Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.International Journal of Antimicrobial Agents, 2006
- Sequence-Based Typing of adeB as a Potential Tool To Identify Intraspecific Groups among Clinical Strains of Multidrug-Resistant Acinetobacter baumanniiJournal of Clinical Microbiology, 2005