Unmethlated domains in vertebrate DNA
Open Access
- 11 February 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (3), 647-658
- https://doi.org/10.1093/nar/11.3.647
Abstract
We have detected a fraction that is rich in unmethylated HpaII and Hhal sites by end-abelling HpaII fragments of chicken DNA. The fraction is not obvious when DNA fragments are stained with ethidium bromide as it amounts to less than 2% of the genome. The average frequency of sites for HpaII is over thirteen times greater in the unmethylated fraction than in total DNA. Partial digests indicate that the unmethylated sites are clustered in the genome. Similar unmethylated fractions were detected in six other vertebrates in both somatic and germ line DNA.Keywords
This publication has 28 references indexed in Scilit:
- Specific DNA methylation sites in the vicinity of the chicken β-globin genesNature, 1979
- Methylation of Mouse Liver DNA Studied by Means of the Restriction Enzymes Msp I and Hpa IIScience, 1979
- The nucleotide sequence of oocyte 5S DNA in xenopus laevis. I. The AT-rich spacerCell, 1978
- The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich regionCell, 1978
- Use of restriction enzymes to study eukaryotic DNA methylationJournal of Molecular Biology, 1978
- MspI, an isoschizomer of HpaII which cleaves both unmethylated and methylated HpaII sitesNucleic Acids Research, 1978
- Analysis of Calf‐Thymus Satellite DNA: Evidence for Specific Methylation of Cytosine in C‐G SequencesEuropean Journal of Biochemistry, 1977
- Unmethylated DNA in mouse L cells: I. DNA fiber autoradiographyBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresisBiochemistry, 1975
- Methylation of DNA in developing sea urchin embryosJournal of Molecular Biology, 1968