Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries
- 3 February 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Protocols
- Vol. 6 (2), 242-251
- https://doi.org/10.1038/nprot.2010.181
Abstract
No abstract availableKeywords
This publication has 20 references indexed in Scilit:
- Toward Engineering Synthetic Microbial MetabolismJournal of Biomedicine and Biotechnology, 2009
- Circular Polymerase Extension Cloning of Complex Gene Libraries and PathwaysPLOS ONE, 2009
- One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genomeProceedings of the National Academy of Sciences of the United States of America, 2008
- DNA assembler, an in vivo genetic method for rapid construction of biochemical pathwaysNucleic Acids Research, 2008
- Harnessing homologous recombination in vitro to generate recombinant DNA via SLICNature Methods, 2007
- Novel methods for cloning and engineering genes using the polymerase chain reactionCurrent Opinion in Biotechnology, 1995
- Recursive PCR: a novel technique for total gene synthesis"Protein Engineering, Design and Selection", 1992
- Rapid and efficient cloning of Alu-PCR products using uracil DNA glycosylase.Genome Research, 1991
- Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extensionGene, 1989
- Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic SequencesScience, 1986