Pinocytosis by Human Alveolar Macrophages COMPARISON OF SMOKERS AND NONSMOKERS
Open Access
- 1 August 1974
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 54 (2), 247-251
- https://doi.org/10.1172/jci107759
Abstract
Alveolar macrophages were obtained from human volunteers, smokers and nonsmokers, by bronchial lavage through a fiberoptic bronchoscope. Cells were incubated in a chemically defined medium containing [14C]sucrose (0.36 mM) and varying concentrations of rabbit serum. Pinocytosis was assessed by the cellular uptake of isotope over 30, 75, and 120-min periods. Pinocytic activity of smokers' cells was dependent on serum concentration but always less than the activity of nonsmokers' cells. The degree of pinocytosis by nonsmokers' cells was independent of serum concentration. It is concluded that the decreased level of pinocytic activity in smokers' alveolar macrophages as indicated by the uptake of sucrose in the presence of rabbit serum may represent a form of reticuloendothelial blockade.Keywords
This publication has 26 references indexed in Scilit:
- Altered Morphology and Increased Acid Hydrolase Content of Pulmonary Macrophages from Cigarette Smokers1–4American Review of Respiratory Disease, 1973
- Low Yield of Pulmonary Surfactant in Cigarette SmokersNew England Journal of Medicine, 1972
- ENDOCYTOSIS IN CHANG LIVER CELLSThe Journal of cell biology, 1971
- Alveolar macrophages. Structural and functional differences between nonsmokers and smokers of marijuana and tobacco.1971
- Ion and macromolecular transport in the alveolar macrophageBiochimica et Biophysica Acta (BBA) - Biomembranes, 1971
- The human alveolar macrophage: isolation, cultivation in vitro, and studies of morphologic and functional characteristicsJCI Insight, 1971
- CIGARETTE SMOKING AND ACUTE NON-INFLUENZAL RESPIRATORY DISEASE IN MILITARY CADETSAmerican Journal of Epidemiology, 1971
- The ultrastructure of alveolar macrophages from human cigarette smokers and nonsmokers.1971
- Pinocytosis as the Cause of Sucrose NephrosisNephron, 1971
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951