A Repressor Protein Complex Regulates Leaf Growth in Arabidopsis
Open Access
- 31 July 2015
- journal article
- Published by Oxford University Press (OUP) in THE PLANT CELL ONLINE
- Vol. 27 (8), 2273-2287
- https://doi.org/10.1105/tpc.15.00006
Abstract
Cell number is an important determinant of final organ size. In the leaf, a large proportion of cells are derived from the stomatal lineage. Meristemoids, which are stem cell-like precursor cells, undergo asymmetric divisions, generating several pavement cells adjacent to the two guard cells. However, the mechanism controlling the asymmetric divisions of these stem cells prior to differentiation is not well understood. Here, we characterized PEAPOD (PPD) proteins, the only transcriptional regulators known to negatively regulate meristemoid division. PPD proteins interact with KIX8 and KIX9, which act as adaptor proteins for the corepressor TOPLESS. D3-type cyclin encoding genes were identified among direct targets of PPD2, being negatively regulated by PPDs and KIX8/9. Accordingly, kix8 kix9 mutants phenocopied PPD loss-of-function producing larger leaves resulting from increased meristemoid amplifying divisions. The identified conserved complex might be specific for leaf growth in the second dimension, since it is not present in Poaceae (grasses), which also lack the developmental program it controls.Keywords
This publication has 62 references indexed in Scilit:
- An improved toolbox to unravel the plant cellular machinery by tandem affinity purification of Arabidopsis protein complexesNature Protocols, 2014
- Direct roles of SPEECHLESS in the specification of stomatal self-renewing cellsScience, 2014
- Stomatal Development in ArabidopsisThe Arabidopsis Book, 2013
- Exit from Proliferation during Leaf Development in Arabidopsis thaliana: A Not-So-Gradual ProcessDevelopmental Cell, 2012
- Diverse roles of Groucho/Tup1 co-repressors in plant growth and developmentPlant Signaling & Behavior, 2012
- The Cyc8–Tup1 complex inhibits transcription primarily by masking the activation domain of the recruiting proteinGenes & Development, 2011
- The JAZ Proteins: A Crucial Interface in the Jasmonate Signaling CascadeTHE PLANT CELL ONLINE, 2011
- Molecular Profiling of Stomatal Meristemoids Reveals New Component of Asymmetric Cell Division and Commonalities among Stem Cell Populations inArabidopsisPlant Cell, 2011
- Groucho-Mediated Repression May Result from a Histone Deacetylase-Dependent Increase in Nucleosome DensityPLOS ONE, 2010
- Solution Structure of the KIX Domain of CBP Bound to the Transactivation Domain of CREB: A Model for Activator:Coactivator InteractionsCell, 1997