Inhibition Of Tumorigenesis in ApcMin/+ Mice by a Combination of (–)-Epigallocatechin-3-gallate and Fish Oil
- 16 August 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 55 (19), 7695-7700
- https://doi.org/10.1021/jf071004r
Abstract
The effect of a combination of (–)-epigallocatechin-3-gallate (EGCG) with fish oil on intestinal tumorigenesis in ApcMin/+ mice fed a high-fat diet was investigated in the present study. The combined treatment of EGCG and fish oil for 9 weeks reduced the tumor number by 53% as compared to controls while neither agent alone had a significant effect. Apoptosis was significantly increased in all treatment groups. β-Catenin nuclear positivity in adenomas from the combination group was lower than control mice, implicating the modulation of Wnt signaling by the combination. Fish oil and the combination significantly reduced prostaglandin E2 (PGE2) levels in small intestinal tumors as compared to controls, suggesting modulation of aberrant arachidonic acid metabolism by fish oil. Akt phosphorylation in adenomas was significantly reduced in all treatment groups, which may have contributed to the observed increase in apoptosis. The results indicate that a combination of low doses of EGCG and fish oil can inhibit tumor multiplicity in ApcMin/+ mice.Keywords
This publication has 33 references indexed in Scilit:
- Green tea polyphenol (−)‐epigallocatechin‐3‐gallate inhibits cyclooxygenase‐2 expression in colon carcinogenesisMolecular Carcinogenesis, 2006
- Mechanisms of omega-3 fatty acid-induced growth inhibition in MDA-MB-231 human breast cancer cellsBreast Cancer Research and Treatment, 2005
- (−)-Epigallocatechin Gallate and Polyphenon E Inhibit Growth and Activation of the Epidermal Growth Factor Receptor and Human Epidermal Growth Factor Receptor-2 Signaling Pathways in Human Colon Cancer CellsClinical Cancer Research, 2005
- Cardiovascular Events Associated with Rofecoxib in a Colorectal Adenoma Chemoprevention TrialNew England Journal of Medicine, 2005
- Cardiovascular Risk Associated with Celecoxib in a Clinical Trial for Colorectal Adenoma PreventionNew England Journal of Medicine, 2005
- Chemoprevention of colorectal cancer: an updateTechniques in Coloproctology, 2004
- β-catenin-mediated signaling: a molecular target for early chemopreventive interventionMutation Research, 2004
- Epigallocatechin-3-gallate Inhibits Epidermal Growth Factor Receptor Signaling PathwayJournal of Biological Chemistry, 2004
- Response of Apcmin and A33ΔNβ-cat mutant mice to treatment with tea, sulindac, and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)Mutation Research, 2002
- The Min (multiple intestinal neoplasia) mutation: its effect on gut epithelial cell differentiation and interaction with a modifier system.The Journal of cell biology, 1992