Functional Analysis of Transcription Factors in Arabidopsis
- 28 May 2009
- journal article
- review article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 50 (7), 1232-1248
- https://doi.org/10.1093/pcp/pcp075
Abstract
Transcription factors (TFs) regulate the expression of genes at the transcriptional level. Modification of TF activity dynamically alters the transcriptome, which leads to metabolic and phenotypic changes. Thus, functional analysis of TFs using 'omics-based' methodologies is one of the most important areas of the post-genome era. In this mini-review, we present an overview of Arabidopsis TFs and introduce strategies for the functional analysis of plant TFs, which include both traditional and recently developed technologies. These strategies can be assigned to five categories: bioinformatic analysis; analysis of molecular function; expression analysis; phenotype analysis; and network analysis for the description of entire transcriptional regulatory networks.Keywords
This publication has 135 references indexed in Scilit:
- Small RNA metabolism in ArabidopsisTrends in Plant Science, 2008
- The microRNA regulated SBP-box genes SPL9 and SPL15 control shoot maturation in ArabidopsisPlant Molecular Biology, 2008
- Regulation of LANCEOLATE by miR319 is required for compound-leaf development in tomatoNature Genetics, 2007
- Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesisProceedings of the National Academy of Sciences, 2007
- Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expressionProceedings of the National Academy of Sciences, 2006
- Using RNA sample titrations to assess microarray platform performance and normalization techniquesNature Biotechnology, 2006
- A subset of Arabidopsis AP2 transcription factors mediates cytokinin responses in concert with a two-component pathwayProceedings of the National Academy of Sciences, 2006
- A gene expression map of Arabidopsis thaliana developmentNature Genetics, 2005
- Differential regulation of EIN3 stability by glucose and ethylene signalling in plantsNature, 2003
- Statistical significance for genomewide studiesProceedings of the National Academy of Sciences, 2003