Antibacterial Effects of Garlic Extracts and Ziziphora Essential Oil on Bacteria Associated with Peri-Implantitis
Open Access
- 1 April 2017
- journal article
- Published by JCDR Research and Publications in Journal of Clinical and Diagnostic Research
- Vol. 11 (4), ZC16-ZC19
- https://doi.org/10.7860/JCDR/2017/24786.9620
Abstract
Peri-implantitis is an inflammatory process, characterized by loss of bone around implants and bleeding on probing. Colonization of bacteria in the pocket created around the implant is one of the most important aetiologic factors involved. Although antibiotics are routinely used and are effective agents against this bacterial infection, due to the side effects and drug resistance of these medications, researchers are more interested in using herbal derivatives. There are relatively limited number of studies in this respect. To evaluate and compare the antibacterial effects of the extracts of Allium sativum and Ziziphora clinopodioides essential oil on S. aureus and P. aeruginosa. In this in vitro study conducted at Tabriz University of Medical Sciences between March 2016 and July 2016, aqueous and methanolic extracts of garlic and ziziphora essential oil were prepared and then their effects on one standard strain of P. aeruginosa and two standard strains of S. aureus and 18 clinical strains, (nine strains of P. aeruginosa and nine strains of S. aureus) which had been isolated from wound and blood cultures, were evaluated using the reference broth macro dilution method and disk diffusion technique. Data were evaluated with descriptive statistical techniques and t-test for independent groups, using SPSS 17. Aqueous and methanolic extracts of garlic did not exhibit inhibitory effects on S. aureus and P. aeruginosa. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for ziziphora essential oil against S. aureus were 0.25 and 0.5 μg/mL, respectively. The mean diameter of growth inhibition zone for S. aureus in the ziziphora essential oil group (0.25μg/mL) and Vancomycin group (10 μg/mL) were 26.09±5.8 and 14.63±1.20 mm respectively (p<0.001). Growth inhibition zone for P. aeruginosa were observed in only one (35 mm) and three cases (12.5±3.4 mm) in the ziziphora essential oil group (0.5 μg/mL) and Nalidixic acid group (10 μg/mL) respectively. The essential oil of ziziphora exhibited a favourable antibacterial effect on S. aureus; therefore, its extract can be used as an effective antimicrobial agent alone or in association with other antibiotics in the treatment of infections due to S. aureus.Keywords
This publication has 10 references indexed in Scilit:
- The epidemiology of peri‐implantitisClinical Oral Implants Research, 2012
- Prevalence of peri‐implant diseases. A cross‐sectional study based on a private practice environmentJournal of Clinical Periodontology, 2012
- Inhibitory effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistant pathogenic bacteriaAnnals of Clinical Microbiology and Antimicrobials, 2012
- Peri-implantitis: Associated microbiota and treatmentMedicina Oral Patología Oral y Cirugia Bucal, 2011
- Antimicrobial Effect of Garlic (Allium sativum)Recent Patents on Anti-Infective Drug Discovery, 2010
- Extent of peri‐implantitis‐associated bone lossJournal of Clinical Periodontology, 2009
- Biomaterial and antibiotic strategies for peri‐implantitis: A reviewJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2008
- Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substancesNature Protocols, 2008
- Antibacterial Activity and Composition of the Essential Oil of Ziziphora clinopodioides subsp. bungeana (Juz.) Rech. f. from IranZeitschrift für Naturforschung C, 2006
- Essential Oil Composition, Antibacterial and Antioxidant Activity of the Oil and Various Extracts of Ziziphora clinopodioides subsp. rigida (BOISS.) RECH. f. from IranBiological & Pharmaceutical Bulletin, 2005