Metallo-beta-Lactamase VIM-1, SPM-1, and IMP-1 Genes Among Clinical Pseudomonas aeruginosa Species Isolated in Zahedan, Iran
Open Access
- 18 April 2015
- journal article
- Published by Briefland in Jundishapur Journal of Microbiology
- Vol. 8 (4), e17489
- https://doi.org/10.5812/jjm.8(4)2015.17489
Abstract
Background: One of the major clinical problems regarding Pseudomonas aeruginosa is attributed to metallo-beta-lactamases (MBL). This group of enzymes is a subset of beta lactamases which belong to group B of Ambler classification and cause hydrolysis of carbapenems. Based on epidemiological studies conducted worldwide, it is proved that prevalence of genes coding MBLs in P. aeruginosa species are different in various geographic zones and even in various hospitals. Therefore, according to the clinical importance of organisms generating MBLs, it is necessary to identify and control these bacteria in hospitals for therapeutic purposes. Objectives: The current study aimed to investigate the Metallo-beta-Lactamase VIM-1, SPM-1, and IMP-1 genes among clinical P. aeruginosa species isolated in Zahedan, Iran. Materials and Methods: The current study investigated the presence of MBL through phenotypic and genotypic methods and also the pattern of antibiotic resistance in P. aeruginosa species isolated in hospitals. The Minimum Inhibitory Concentration (MIC) against imipeneme was measured for 191 P. aeruginosa species isolated from Zahedan hospitals after identification through biochemical methods and determination of the antibiotic resistance pattern. Strains with MIC > 4 µg/mL were studied by phenotypic and genotypic methods. Results: The rate of resistance against imipeneme was 5.7% and after carrying out the phenotypic experiments, nine species were identified as of MBL producer. Seven species were confirmed by Polymerase Chain Reaction (PCR) method. Gene VIM-1 was the predominant gene among the positive (antibiotic resistant) species. Conclusions: The study results showed that MBL genes were present in some of the species isolated from Zahedan hospitals. Regarding the importance of MBL producer bacteria in hospitals, quick identification and evaluation of these clinical species can be considered as an important and basic step for treatment and control of pseudomonad infections. Keywords: Metallo-beta-lactamase; Pseudomonas aeruginosa; Imipeneme; Antibiotic ResistanceKeywords
This publication has 25 references indexed in Scilit:
- Assessment of extended-spectrum β-lactamase, KPC carbapenemase and porin resistance mechanisms in clinical samples of Klebsiella pneumoniae and Enterobacter spp.International Journal of Antimicrobial Agents, 2013
- Characterization of the New Metallo-β-Lactamase VIM-13 and Its Integron-Borne Gene from a Pseudomonas aeruginosa Clinical Isolate in SpainAntimicrobial Agents and Chemotherapy, 2008
- Synergistic Effect of Thymol and Carvacrol Combined with Chelators and Organic Acids against Salmonella TyphimuriumJournal of Food Protection, 2007
- The peculiarities of Pseudomonas aeruginosa resistance to antibiotics and prevalence of serogroupsMedicina, 2007
- Molecular Evolution of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa in a Nosocomial Setting of High-Level EndemicityJournal of Clinical Microbiology, 2006
- Estimation of efflux mediated multi-drug resistance and its correlation with expression levels of two major efflux pumps in mycobacteria.2006
- Molecular Epidemiology of Acquired-Metallo-β-Lactamase-Producing Bacteria in PolandAntimicrobial Agents and Chemotherapy, 2006
- Novel Acquired Metallo-β-Lactamase Gene,blaSIM-1, in a Class 1 Integron fromAcinetobacter baumanniiClinical Isolates from KoreaAntimicrobial Agents and Chemotherapy, 2005
- The Metallo-?-Lactamases Fall into Two Distinct Phylogenetic GroupsJournal of Molecular Evolution, 2003
- Comparing antimicrobial activity against resistant Pseudomonas aeruginosa using an index for the absence of cross-resistanceJournal of Antimicrobial Chemotherapy, 1995