Characterization of a new peptide fromTityus serrulatusscorpion venom which is a ligand of the apamin-binding site
- 15 July 1996
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 390 (1), 81-84
- https://doi.org/10.1016/0014-5793(96)00616-3
Abstract
A new ligand (Ts κ) of the apamin binding site on rat brain synaptosomes (K 0.5 = 300 pM) was purified and characterized from the venom of Tityus serrulatus. It is a polypeptide toxin of 35 amino acid residues, with three disulfide bridges. Its cDNA was amplified from a venom gland cDNA library and the nucleotide sequence determined. A model of Ts κ was constructed by amino acid replacement using charybdotoxin structure as determined by 1H nuclear magnetic resonance as starting model.Keywords
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