Structural characterization and identification of C19‐ and C20‐diterpenoid alkaloids in roots of Aconitum carmichaeli by rapid‐resolution liquid chromatography coupled with time‐of‐flight mass spectrometry
- 30 April 2009
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 23 (11), 1619-1635
- https://doi.org/10.1002/rcm.4038
Abstract
Aconite alkaloids from the roots of Aconitum carmichaeli (Fuzi, in Chinese) have been investigated by rapid‐resolution liquid chromatography coupled with time‐of‐flight mass spectrometry (TOFMS) in positive mode. With dynamic adjustment of the key role as fragmentor voltage in TOFMS, an efficient transmission of the ions was achieved to obtain the best sensitivity for providing the molecular formula for each analyte, and abundant fragment ions for structural information. Fifteen authentic standards isolated from Fuzi with various structures were first characterized by TOFMS, including diester‐diterpenoid alkaloids (DDAs), monoester‐diterpenoid alkaloids (MDAs), alkylol amine‐diterpenoid alkaloids (ADAs), veatchine‐type alkaloids and atisine‐type alkaloids. Fragmentation rules and key diagnostic fragment ions have been summarized, and possible pathways of fragmentation have been proposed. By accurate mass measurements within 5 ppm error for each ion, 30 C19‐diterpenoid alkaloids including 10 DDAs, 3 MDAs, 9 ADAs and 8 other type alkaloids, and 8 C20‐diterpenoid alkaloids including 4 veatchine‐type alkaloids and 4 atisine‐type alkaloids could be identified in a methanolic extract of Fuzi. Some isomers of aconite alkaloids were also differentiated. Based on the differences between their fragmentation pathways and special fragment ions, each type of aconite alkaloids was differentiated. Copyright © 2009 John Wiley & Sons, Ltd.Keywords
Funding Information
- The National Science Foundation and National Key Technologies R&D Program of China (30530870)
This publication has 24 references indexed in Scilit:
- Studies on the aconitine-type alkaloids in the roots of Aconitum Carmichaeli Debx. by HPLC/ESIMS/MSnTalanta, 2009
- Alkaloid profiling of the Chinese herbal medicine Fuzi by combination of matrix-assisted laser desorption ionization mass spectrometry with liquid chromatography–mass spectrometryJournal of Chromatography A, 2009
- Studies on the stability of diester-diterpenoid alkaloids from the genus Aconitum L. by high performance liquid chromatography combined with electrospray ionisation tandem mass spectrometry (HPLC/ESI/MSn )Phytochemical Analysis, 2007
- Investigation of Aconitine-type Alkaloids from Processed Tuber of Aconitum carmiechaeli by HPLC-ESI-MS/MSnChemical Research in Chinese Universities, 2007
- High‐speed analyses using rapid resolution liquid chromatography on 1.8‐μm porous particlesJournal of Separation Science, 2006
- Sensitive analysis of aconitine, hypaconitine, mesaconitine and jesaconitine in human body fluids and Aconitum tubers by LC/ESI‐TOF‐MSJournal of Mass Spectrometry, 2006
- Simultaneous determination of six Aconitum alkaloids in proprietary Chinese medicines by high-performance liquid chromatographyJournal of Chromatography A, 2005
- Comparison of the antinociceptive action of crude Fuzei, the root of Aconitum, and its processed productsJournal of Ethnopharmacology, 2005
- Quantitation and Accurate Mass Analysis of Pesticides in Vegetables by LC/TOF-MSAnalytical Chemistry, 2005
- Characterization of aconitine‐type alkaloids in the flowers of Aconitum kusnezoffii by electrospray ionization tandem mass spectrometryJournal of Mass Spectrometry, 2003