A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activity
- 1 January 1964
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 90 (1), 147-149
- https://doi.org/10.1042/bj0900147
Abstract
32p-labelled ATP of high specific activity is prepared by an exchange reaction. The method requires only substrates and enzymes that are available commercially. Hydrolysis of the labelled ATP with "heavy" meromyosin indicates that 98-99% of the 32p is in the gamma-phosphate group.This publication has 4 references indexed in Scilit:
- The degradation of heavy meromyosin by trypsinBiochemical Journal, 1962
- Mechanisms of Synthesis of Adenosine TriphosphatePublished by Wiley ,1961
- Partial resolution of the enzymes catalyzing oxidative phosphorylation. II. Participation of a soluble adenosine tolphosphatase in oxidative phosphorylation.1960
- Preparation of [β-32P] ATP and [γ-32P] ATPBiochimica et Biophysica Acta, 1958