Elicitor-induced transcription factors for metabolic reprogramming of secondary metabolism in Medicago truncatula
Open Access
- 22 December 2008
- journal article
- Published by Springer Science and Business Media LLC in BMC Plant Biology
- Vol. 8 (1), 132
- https://doi.org/10.1186/1471-2229-8-132
Abstract
Exposure of Medicago truncatula cell suspension cultures to pathogen or wound signals leads to accumulation of various classes of flavonoid and/or triterpene defense molecules, orchestrated via a complex signalling network in which transcription factors (TFs) are essential components. In this study, we analyzed TFs responding to yeast elicitor (YE) or methyl jasmonate (MJ). From 502 differentially expressed TFs, WRKY and AP2/EREBP gene families were over-represented among YE-induced genes whereas Basic Helix-Loop-Helix (bHLH) family members were more over-represented among the MJ-induced genes. Jasmonate ZIM-domain (JAZ) transcriptional regulators were highly induced by MJ treatment. To investigate potential involvement of WRKY TFs in signalling, we expressed four Medicago WRKY genes in tobacco. Levels of soluble and wall bound phenolic compounds and lignin were increased in all cases. WRKY W109669 also induced tobacco endo-1,3-β-glucanase (NtPR2) and enhanced the systemic defense response to tobacco mosaic virus in transgenic tobacco plants. These results confirm that Medicago WRKY TFs have broad roles in orchestrating metabolic responses to biotic stress, and that they also represent potentially valuable reagents for engineering metabolic changes that impact pathogen resistance.Keywords
This publication has 90 references indexed in Scilit:
- A transcript profiling approach reveals an epicatechin-specific glucosyltransferase expressed in the seed coat of Medicago truncatulaProceedings of the National Academy of Sciences of the United States of America, 2008
- Metabolomics Reveals Novel Pathways and Differential Mechanistic and Elicitor-Specific Responses in Phenylpropanoid and Isoflavonoid Biosynthesis in Medicago truncatula Cell CulturesPlant Physiology, 2007
- Different mechanisms for phytoalexin induction by pathogen and wound signals in Medicago truncatulaProceedings of the National Academy of Sciences, 2007
- A Downstream Mediator in the Growth Repression Limb of the Jasmonate PathwayPlant Cell, 2007
- Networks of WRKY transcription factors in defense signalingCurrent Opinion in Plant Biology, 2007
- Lignin modification improves fermentable sugar yields for biofuel productionNature Biotechnology, 2007
- Legume Transcription Factors: Global Regulators of Plant Development and Response to the EnvironmentPlant Physiology, 2007
- Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevinesBMC Plant Biology, 2007
- Systematic functional analysis of the Caenorhabditis elegans genome using RNAiNature, 2003
- Lignification as a Mechanism of Disease ResistanceAnnual Review of Phytopathology, 1980