Comparative proteomic analysis of plasma membrane proteins between human osteosarcoma and normal osteoblastic cell lines
Open Access
- 14 May 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Cancer
- Vol. 10 (1), 206
- https://doi.org/10.1186/1471-2407-10-206
Abstract
Osteosarcoma (OS) is the most common primary malignant tumor of bone in children and adolescents. However, the knowledge in diagnostic modalities has progressed less. To identify new biomarkers for the early diagnosis of OS as well as for potential novel therapeutic candidates, we performed a sub-cellular comparative proteomic research. An osteosarcoma cell line (MG-63) and human osteoblastic cells (hFOB1.19) were used as our comparative model. Plasma membrane (PM) was obtained by aqueous two-phase partition. Proteins were analyzed through iTRAQ-based quantitative differential LC/MS/MS. The location and function of differential proteins were analyzed through GO database. Protein-protein interaction was examined through String software. One of differentially expressed proteins was verified by immunohistochemistry. 342 non-redundant proteins were identified, 68 of which were differentially expressed with 1.5-fold difference, with 25 up-regulated and 43 down-regulated. Among those differential proteins, 69% ware plasma membrane, which are related to the biological processes of binding, cell structure, signal transduction, cell adhesion, etc., and interaction with each other. One protein--CD151 located in net nodes was verified to be over-expressed in osteosarcoma tissue by immunohistochemistry. It is the first time to use plasma membrane proteomics for studying the OS membrane proteins according to our knowledge. We generated preliminary but comprehensive data about membrane protein of osteosarcoma. Among these, CD151 was further validated in patient samples, and this small molecule membrane might be a new target for OS research. The plasma membrane proteins identified in this study may provide new insight into osteosarcoma biology and potential diagnostic and therapeutic biomarkers.Keywords
This publication has 43 references indexed in Scilit:
- Proteomic Analysis of Chemonaïve Pediatric Osteosarcomas and Corresponding Normal Bone Reveals Multiple Altered Molecular TargetsJournal of Proteome Research, 2009
- An integrative multi-platform analysis for discovering biomarkers of osteosarcomaBMC Cancer, 2009
- Proteomics of the lysosomeBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2009
- In-Depth Proteomics to Define the Cell Surface and Secretome of Ovarian Cancer Cells and Processes of Protein SheddingCancer Research, 2009
- Global Protein-expression Analysis of Bone and Soft Tissue SarcomasClinical Orthopaedics and Related Research, 2008
- The proteome profile of the human osteosarcoma U2OS cell line.2008
- Therapy for OsteosarcomaPediatric Drugs, 2008
- Adjuvant and neoadjuvant combination chemotherapy for osteogenic sarcomaCurrent Opinion in Oncology, 2007
- Comparative proteomic analysis of human osteosarcoma and SV40-immortalized normal osteoblastic cell linesActa Pharmacologica Sinica, 2007
- Survival Data for 648 Patients with Osteosarcoma Treated at One InstitutionClinical Orthopaedics and Related Research, 2004