Amperometric carbon paste enzyme electrodes with Fe3O4nanoparticles and 1,4-Benzoquinone for glucose determination
- 19 July 2012
- journal article
- research article
- Published by Taylor & Francis Ltd in Artificial Cells, Nanomedicine, and Biotechnology
- Vol. 41 (3), 165-171
- https://doi.org/10.3109/10731199.2012.712045
Abstract
Two new amperometric carbon paste enzyme electrodes including Fe3O4 nanoparticles with and without 1,4-benzoquinone were developed for glucose determination. Electron transfer properties of unmodified and Fe3O4 nanoparticles and/or 1,4-benzoquinone modified carbon paste electrodes were investigated in 0.1 M KCl support electrolyte containing Fe(CN)63−/4− as redox probe by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods. Fe3O4 nanoparticles increased electron transfer at solution/electrode interface. The parameters affecting the analytical performance of the enzyme electrode have been investigated in detail and optimized for Fe3O4 nanoparticle modified enzyme electrode (Fe3O4–CPEE). Fe3O4 nanoparticles and 1,4-benzoquinone modified enzyme electrode (BQ‐Fe3O4‐CPEE) exhibited linear response from 1.9 × 10‐7 M to 3.7 × 10‐6 M, from 7.2 × 10‐6 M to 1.5 × 10‐4 M and from 1.3 × 10‐3 M to 1.2 × 10‐2 M with an excellent detection limit of 1.9 × 10‐8 M. BQ‐Fe3O4‐CPEE was used for determination of glucose in serum samples and results were in good agreement with those obtained by spectrophotometric method.Keywords
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