Alzheimer's β‐amyloid peptide specifically interacts with and is degraded by insulin degrading enzyme
- 23 May 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 345 (1), 33-37
- https://doi.org/10.1016/0014-5793(94)00387-4
Abstract
Cerebral deposition of β-amyloid peptide (βA) is a hallmark of Alzheimer's disease. Concentration of βA could play a critical role in the rate of amyloid deposition. It is therefore of considerable importance to identify proteases involved in processing of βA. 125I-labeled synthetic βA specifically cross-linked to a single protein with M r = 110,000 in cytosol fractions from rat brain and liver. This protein was identified as insulin degrading enzyme (IDE) since the labeling of the 110 kDa protein was completely blocked by an excess of insulin, and anti-IDE monoclonal antibodies precipitated the labeled protein. Purified rat IDE effectively degraded βA.Keywords
This publication has 29 references indexed in Scilit:
- Isolation and quantification of soluble Alzheimer's β-peptide from biological fluidsNature, 1992
- Amyloid β-peptide is produced by cultured cells during normal metabolismNature, 1992
- Solution conformations and aggregational properties of synthetic amyloid β-peptides of Alzheimer's disease: Analysis of circular dichroism spectraJournal of Molecular Biology, 1992
- Targeting of cell-surface β-amyloid precursor protein to lysosomes: alternative processing into amyloid-bearing fragmentsNature, 1992
- Processing of the Amyloid Protein Precursor to Potentially Amyloidogenic DerivativesScience, 1992
- Atrial natriuretic peptide (ANP) is a high‐affinity substrate for rat insulin‐degrading enzymeJBIC Journal of Biological Inorganic Chemistry, 1991
- Atrial natriuretic peptide deposited as atrial amyloid fibrilsBiochemical and Biophysical Research Communications, 1987
- Characterization and Chromosomal Localization of a cDNA Encoding Brain Amyloid of Alzheimer's DiseaseScience, 1987
- Alzheimer's disease: Initial report of the purification and characterization of a novel cerebrovascular amyloid proteinBiochemical and Biophysical Research Communications, 1984
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970