Immunological properties of FMDV-gP64 fusion proteins expressed on SF9 cell and baculovirus surfaces
- 21 December 2004
- journal article
- Published by Elsevier BV in Vaccine
- Vol. 23 (6), 840-845
- https://doi.org/10.1016/j.vaccine.2004.03.070
Abstract
In the present report, we characterized the immune response and the protection conferred by recombinant baculoviruses or infected insect cells expressing the fusions gp64-P1 and gp64-site A FMDV antigens. Mice, vaccinated intraperitoneally with gp64-P1 immunogens, showed a low-antibody response and a variable degree of protection. However, when mice received recombinant baculoviruses or infected insect cells expressing the fusion protein gp64-site A, high-ELISA and seroneutralizing titers (SNT) against FMDV were elicited. All mice immunized with Sf9 cells expressing FMDV site A developed a protective immune response against challenge with virulent FMDV, indicating that the baculovirus display of foreign epitopes is a promising approach to biosynthetic vaccines.Keywords
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