Baicalin’s Therapeutic Time Window of Neuroprotection during Transient Focal Cerebral Ischemia and Its Antioxidative EffectsIn VitroandIn Vivo
Open Access
- 25 June 2013
- journal article
- research article
- Published by Hindawi Limited in Evidence-Based Complementary and Alternative Medicine
- Vol. 2013, 1-11
- https://doi.org/10.1155/2013/120261
Abstract
We investigated the effects of baicalin on an ischemia-reperfusion-induced brain injury model in rats and its antioxidative activitiesin vitroandin vivo. An ischemia-reperfusion injury of the brain via a middle cerebral artery occlusion (MCAO) was induced in rats. Baicalin was injected at different time points (0, 2, 4, and 6 h) after the MCAO was induced. Baicalin can improve neurological function and significantly decrease brain infarction within a time window of 4 h. Moreover, baicalin was able to reduce cell apoptosis and had the strong antioxidative effect of reducing reactive oxygen species production and malondialdehyde generation. In contrast, baicalin interfered with superoxide dismutase and nicotinamide adenine dinucleotide 2′-phosphate oxidase activities. Moreover, baicalin also exhibited strong neuroprotective effects against H2O2-mediated injury and improved the SOD activity of neurons. Furthermore, baicalin demonstrated good scavenging of hydroxyl radicals, superoxide anions, and DPPH radicals and exerted an additional effect of inhibiting xanthine oxidase. Baicalin showed beneficial effects against MCAO-induced injury within a 4 h time window, and its antioxidative effects bothin vitroandin vivomay partly elucidate its mechanism of action.Keywords
Funding Information
- National Science and Technology Major Projects (2009ZX09102-136)
This publication has 46 references indexed in Scilit:
- Refined Qingkailing Protects MCAO Mice from Endoplasmic Reticulum Stress-Induced Apoptosis with a Broad Time WindowEvidence-Based Complementary and Alternative Medicine, 2012
- NADPH oxidase is the primary source of superoxide induced by NMDA receptor activationNature Neuroscience, 2009
- Update of the Stroke Therapy Academic Industry Roundtable Preclinical RecommendationsStroke, 2009
- NMDA Receptor Activation Increases Free Radical Production through Nitric Oxide and NOX2Journal of Neuroscience, 2009
- Perivascular nitric oxide and superoxide in neonatal cerebral hypoxia-ischemiaAmerican Journal of Physiology-Heart and Circulatory Physiology, 2008
- Antioxidative properties of galantamine on neuronal damage induced by hydrogen peroxide in SK–N–SH cellsNeuroToxicology, 2008
- Baicalin inhibits macrophage activation by lipopolysaccharide and protects mice from endotoxin shockBiochemical Pharmacology, 2007
- Ethanol preconditioning protects against ischemia/reperfusion-induced brain damage: Role of NADPH oxidase-derived ROSFree Radical Biology & Medicine, 2007
- Antioxidative Activity of Volatile Extracts Isolated from Angelica tenuissimae Roots, Peppermint Leaves, Pine Needles, and Sweet Flag LeavesJournal of Agricultural and Food Chemistry, 2005
- Neurological Deficit and Extent of Neuronal Necrosis Attributable to Middle Cerebral Artery Occlusion in RatsStroke, 1995