Crystal structure of oxygen-evolving photosystem II from Thermosynechococcus vulcanus at 3.7-Å resolution
- 23 December 2002
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 100 (1), 98-103
- https://doi.org/10.1073/pnas.0135651100
Abstract
Photosystem II (PSII) is a multisubunit membrane protein complex performing light-induced electron transfer and water-splitting reactions, leading to the formation of molecular oxygen. The first crystal structure of PSII from a thermophilic cyanobacterium Thermosynechococcus elongatus was reported recently [Zouni, A., Witt, H. T., Kern, J., Fromme, P., Krauss, N., Saenger, W. & Orth, P. (2001) Nature 409, 739-743)] at 3.8-A resolution. To analyze the PSII structure in more detail, we have obtained the crystal structure of PSII from another thermophilic cyanobacterium, Thermosynechococcus vulcanus, at 3.7-A resolution. The present structure was built on the basis of the sequences of PSII large subunits D1, D2, CP47, and CP43; extrinsic 33- and 12-kDa proteins and cytochrome c550; and several low molecular mass subunits, among which the structure of the 12-kDa protein was not reported previously. This yielded much information concerning the molecular interactions within this large protein complex. We also show the arrangement of chlorophylls and cofactors, including two beta-carotenes recently identified in a region close to the reaction center, which provided important clues to the secondary electron transfer pathways around the reaction center. Furthermore, possible ligands for the Mn-cluster were determined. In particular, the C terminus of D1 polypeptide was shown to be connected to the Mn cluster directly. The structural information obtained here provides important insights into the mechanism of PSII reactions.This publication has 50 references indexed in Scilit:
- Localisation of the PsbH subunit in photosystem II: a new approach using labelling of his-tags with a Ni2+-NTA gold cluster and single particle analysisJournal of Molecular Biology, 2001
- P680, the primary electron donor of photosystem IIJournal of Photochemistry and Photobiology A: Chemistry, 2001
- High-resolution structure of the OmpA membrane domainJournal of Molecular Biology, 2000
- STRUCTURE AND MEMBRANE ORGANIZATION OF PHOTOSYSTEM II IN GREEN PLANTSAnnual Review of Plant Physiology and Plant Molecular Biology, 1997
- Refinement of Macromolecular Structures by the Maximum-Likelihood MethodActa crystallographica. Section D, Structural biology, 1997
- 15‐cis‐β‐Carotene found in the reaction center of spinach photosystem IIFEBS Letters, 1995
- A quantitative secondary structure analysis of the 33 kDa extrinsic polypeptide of photosystem II by FTIR spectroscopyFEBS Letters, 1995
- The CCP4 suite: programs for protein crystallographyActa crystallographica. Section D, Structural biology, 1994
- Orientation and nearest neighbor analysis of psbI gene product in the photosystem II reaction center complex using bifunctional cross‐linkersFEBS Letters, 1993
- Stoichiometric association of extrinsic cytochrome c550 and 12 kDa protein with a highly purified oxygen‐evolving photosystem II core complex from Synechococcus vulcanusFEBS Letters, 1992