Adipocyte Fatty Acid–Binding Protein Is a Plasma Biomarker Closely Associated with Obesity and Metabolic Syndrome
- 1 March 2006
- journal article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 52 (3), 405-413
- https://doi.org/10.1373/clinchem.2005.062463
Abstract
Background: Adipocyte fatty acid–binding protein (A-FABP) is traditionally thought to be a cytosolic fatty acid chaperone expressed in adipocytes. Mice with targeted disruption of the A-FABP gene exhibit a striking phenotype with strong protection from insulin resistance, hyperglycemia, and atherosclerosis. The clinical relevance of these findings remains to be confirmed. Methods: We used tandem mass spectrometry–based proteomic analysis to identify proteins secreted from adipocytes and present in human serum. We measured serum A-FABP concentrations in 229 persons (121 men and 108 women; age range, 33–72 years), including 100 lean [body mass index (BMI) 25 kg/m2) selected from a previous cross-sectional study. Results: A-FABP was released from adipocytes and was abundantly present in human serum. Mean (SD) circulating concentrations of A-FABP were significantly higher in overweight/obese than in lean persons [32.3 (14.8) vs 20.0 (9.8) μg/L; P P P Conclusions: A-FABP is a circulating biomarker closely associated with obesity and components of the metabolic syndrome, and measurement of serum concentrations of A-FABP might be useful for clinical diagnosis of obesity-related metabolic and cardiovascular disorders.Keywords
This publication has 49 references indexed in Scilit:
- Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetesNature, 2005
- Proteomic and functional characterization of endogenous adiponectin purified from fetal bovine serumProteomics, 2004
- Combined Adipocyte-Macrophage Fatty Acid–Binding Protein Deficiency Improves Metabolism, Atherosclerosis, and Survival in Apolipoprotein E–Deficient MiceCirculation, 2004
- Atorvastatin reduces CD68, FABP4, and HBP expression in oxLDL-treated human macrophagesBiochemical and Biophysical Research Communications, 2004
- Cytoplasmic fatty acid-binding proteins: emerging roles in metabolism and atherosclerosisCurrent Opinion in Lipidology, 2002
- Sexual dimorphism in circulating leptin concentrations is not accounted for by differences in adipose tissue distributionInternational Journal of Obesity, 2001
- Executive Summary of the Third Report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III)Jama-Journal Of The American Medical Association, 2001
- Improved Glucose and Lipid Metabolism in Genetically Obese Mice Lacking aP2Endocrinology, 2000
- PPARγ Activation Induces the Expression of the Adipocyte Fatty Acid Binding Protein Gene in Human MonocytesBiochemical and Biophysical Research Communications, 1999
- Human intestinal fatty acid binding protein: Report of an assay with studies in normal volunteers and intestinal ischemiaSurgery, 1997