DNA topoisomerase activities in concanavalin A‐stimulated lymphocytes
- 29 October 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 176 (2), 431-435
- https://doi.org/10.1016/0014-5793(84)81212-0
Abstract
Topoisomerase activities have been measured in nuclear extracts of concanavalin A-stimulated lymphocytes. In parallel with the wave of DNA synthesis, type II topoisomerase activity was considerably increased. After 72 h treatment, this activity was stimulated approx. 20-fold over the activity in untreated cells. In contrast, type I topoisomerase was poorly stimulated after 24 h treatment, and 4-5-fold after 72 h. These findings, together with our previous results on regenerating rat liver, suggest a major role of topoisomerase II in DNA replication.Keywords
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