Changes in rat and human pepsinogen phenotypes induced by N′-methyl-N′nitro-n-nitrosoguanidine
- 1 November 1988
- Vol. 62 (9), 1958-1961
- https://doi.org/10.1002/1097-0142(19881101)62:9<1958::aid-cncr2820620915>3.0.co;2-f
Abstract
Changes have been studied in human and rat pepsinogen phenotypes induced by N'‐methyl‐N'nitro‐N‐nitrosoguanidine (MMNG) in in vitro rat experiments and in vivo cultures of human and rat isolated gastric chief cells. In vivo the fastest migrating electrophoretic band decreased or disappeared as early as 3 weeks after the start of MNNG treatment. The changes, observed in 17 of 32 rats receiving MNNG, were permanent and consistently associated with pronounced histopathologic changes seen 10 months later (17 of 17). Comparable phenotypic changes were observed after 7 days only in MNNG‐treated rat chief cell cultures. in human chief cell cultures a decrease of the Pg3 band, which is consistent with the “carcinogenic” phenotype, was observed in two of six preparations treated with MNNG. This early preceding change in phenotype preceding tumor formation may be useful as a diagnostic tool for the onset of gastric cancer.Keywords
This publication has 9 references indexed in Scilit:
- Clinical significance of pepsinogen a isozymogens, serum pepsinogen A and C levels, and serum gastrin levelsCancer, 1987
- Pepsinogen A polymorphism in gastric mucosa and urine, with special reference to patients with gastric cancerClinical Genetics, 1986
- Pepsinogen synthesis in monolayer culture of human and rabbit gastric mucosal cellsVirchows Archiv B Cell Pathology Including Molecular Pathology, 1985
- The influence of omeprazole on the synthesis and secretion of pepsinogen in isolated rabbit gastric glandsBiochemical Pharmacology, 1985
- Variable numbers of pepsinogen genes are located in the centromeric region of human chromosome 11 and determine the high-frequency electrophoretic polymorphism.Proceedings of the National Academy of Sciences of the United States of America, 1985
- Assignment of human pepsinogen A locus to the q12-pter region of chromosome 11Human Genetics, 1985
- Genetics of urinary pepsinogen: A new hypothesisHuman Genetics, 1984
- Changes in Pepsinogen lsozymes in Stomach Cancers Induced in Wistar Rats by N -Methyl- N ′-nitro- N -nitrosoguanidine and in Transplantable Gastric Carcinoma (SG2B) 2JNCI Journal of the National Cancer Institute, 1977
- The pepsinogens of human gastric mucosaGut, 1973