Increase in insulin release from rat pancreatic islets by quinolone antibiotics

Abstract

1 The present study was undertaken to elucidate the mechanism(s) of hypoglycaemia caused by quinolone antibiotics. We investigated the effects of various quinolone antibiotics on insulin release in rat pancreatic islets. 2 At a non-stimulatory concentration of 3 mM glucose, lomefloxacin (LFLX) or sparfloxacin at 1 mM and pipemidic acid (0.1-1 mM) induced slight insulin release but tosufloxacin or enoxacin up to 100 μm did not. 3 At the stimulatory concentration of 10 mM glucose, all quinolones augmented insulin release in a dose-dependent manner. LFLX (100 μm) shifted the dose-response curve of glucose-induced insulin release to the left without altering the maximal response. 4 At 10 mM glucose, LFLX (100 μm) increased insulin release augmented by forskolin (5 μm) or 12-0-tetradecanoyl phorbol-13-acetate (100 nM) but not by raising the K⁺ concentration from 6 to 25 mM. 5 Verapamil (50 μm) or diazoxide (50–400 μm) antagonized the insulinotropic effect of LFLX. 6 These data suggest that quinolone antibiotics may cause hypoglycaemia by increasing insulin release via blockade of ATP-sensitive K⁺ channels.