On the Mediation of Inflammatory Reaction in the Human Gallbladder Epithelium
- 1 May 1976
- journal article
- Published by Informa UK Limited in Scandinavian Journal of Gastroenterology
- Vol. 11 (3), 321-328
- https://doi.org/10.1080/00365521.1976.12097113
Abstract
Human gallbladder epithelium was homogenized with a view to maintaining the integrity of subcellular components. In such homogenates, N-acetyl-beta-glucosaminidase, beta-glucuronidase, beta-galactosidase, beta-glucosidase, beta-fucosidase, beta-xylosidase, and acid phosphatase were demonstrated together with phospholipase activity. All the enzymes exhibited structure-linked latency. After discarding cellular debris from the homogenate, remaining subcellular organelles were analytically separated by density gradient centrifugation. After 100,000 g for 1 hour, particles containing acid glycosidases were recovered at a sucrose density of 1.18-1.19, whereas the mitochondrial marker enzyme succinate-reductase accumulated at a density of 1.16. The bulk of sedimentable phospholipase activity was recovered with particles sedimenting at 1.18-1.19. The results are interpreted as indicating that phosphalipase is present in lysosomes of the human gallbladder epithelium. Release of acid hydrolases, in lysosomes of the human gallbladder epithelium. Release of acid hydrolases, in lysosomes of the human gallbladder epithelium. Release of acid hydrolases, particularly phospholipase A, from the gallbladder epithelium is discussed as mediation of an inflammatory reaction in the gallbladder, i.e. cholecystitis.Keywords
This publication has 10 references indexed in Scilit:
- Lysolecithin and Lecithin in the Gallbladder Wall and Bile; Their Possible Roles in the Pathogenesis of Acute CholecystitisScandinavian Journal of Gastroenterology, 1974
- Preparation and Use of Liposomes as Models of Biological MembranesPublished by Springer Science and Business Media LLC ,1974
- Distribution of membrane-confined phospholipases A in the rat hepatocyteBiochimica et Biophysica Acta (BBA) - Biomembranes, 1972
- Studies on the lysis of red cells and bimolecular lipid leaflets by synthetic lysolecithins, lecithins and structural analogsChemistry and Physics of Lipids, 1969
- Rat-liver lysosomal β-glucosidase: A membrane enzymeBiochimica et Biophysica Acta (BBA) - Enzymology, 1968
- Hydrolysis of phospholipids by a lysosomal enzymeJournal of Lipid Research, 1967
- Quantitative Determination of Plasma Phospholipids in Pregnant and Non‐pregnant Women, with Special Reference to LysolecithinActa Medica Scandinavica, 1964
- Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphataseBiochemical Journal, 1961
- [31] GlucuronidasesMethods in Enzymology, 1955
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951