Protein dynamics from single-molecule fluorescence intensity correlation functions
- 2 September 2009
- journal article
- research article
- Published by AIP Publishing in The Journal of Chemical Physics
- Vol. 131 (9), 095102
- https://doi.org/10.1063/1.3212597
Abstract
Fluorescence intensity correlation functions contain information about photophysical and conformational dynamics. We propose and implement a simple procedure to analyze such functions measured in the presence of resonance energy transfer. When there is a separation of time scales and the conformational dynamics is modeled as diffusion in the potential of mean force along the interdye distance, we obtain an analytic expression for the conformationalcorrelation time. This can be used to find the diffusion coefficient describing conformational fluctuations given the photon count rate and equilibrium distribution.This publication has 13 references indexed in Scilit:
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