pH-Controlled Construction of Chitosan/Alginate Multilayer Film: Characterization and Application for Antibody Immobilization
- 16 November 2007
- journal article
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 23 (26), 13046-13052
- https://doi.org/10.1021/la702774a
Abstract
In this work, a chitosan/alginate multilayer film was constructed via layer-by-layer self-assembly and studied by in situ surface plasmon resonance monitoring and contact angle measurements. The results demonstrate that the surface composition of the self-assembled multilayer film can be simply tailored through pH control during the assembly process. The biological property of the assembled film was further characterized via antigen−antibody interactions, showing that the loading capacity of the antibody on the multilayered film and the binding activity of the antigen to the immobilized antibody could be also well-tuned by pH control. This work can provide more scientific insight in the interaction between protein and polymer matrix and render a novel simple approach to build high-performance biointerfaces through pH control for potential applications of highly sensitive immunosensors.Keywords
This publication has 18 references indexed in Scilit:
- Chitin and chitosan: Properties and applicationsProgress in Polymer Science, 2006
- Surface Tailoring for Controlled Protein Adsorption: Effect of Topography at the Nanometer Scale and ChemistryJournal of the American Chemical Society, 2006
- Tuning compliance of nanoscale polyelectrolyte multilayers to modulate cell adhesionBiomaterials, 2005
- Mechanism of the pH-Induced Discontinuous Swelling/Deswelling Transitions of Poly(allylamine hydrochloride)-Containing Polyelectrolyte Multilayer FilmsMacromolecules, 2005
- Bioactive Coatings of Endovascular Stents Based on Polyelectrolyte MultilayersBiomacromolecules, 2003
- pH-Responsive Properties of Multilayered Poly(l-lysine)/Hyaluronic Acid SurfacesBiomacromolecules, 2003
- Micromosaic ImmunoassaysAnalytical Chemistry, 2000
- Self-assembled monolayers: a versatile tool for the formulation of bio-surfacesTrAC Trends in Analytical Chemistry, 2000
- Photoinduced Energy and Electron Transfer Reactions in Lamellar Polyanion/Polycation Thin Films: Toward an Inorganic “Leaf”Journal of the American Chemical Society, 1999
- Fuzzy Nanoassemblies: Toward Layered Polymeric MulticompositesScience, 1997