Molecular cloning of a human DNA repair gene
- 2 August 1984
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 310 (5976), 425-429
- https://doi.org/10.1038/310425a0
Abstract
Cell strains derived from patients having a hereditary disorder associated with defects in repair of DNA damage such as xeroderma pigmentosum1,2 and mutants isolated from established rodent cell lines3,4 provide the tools for genetic and biochemical analysis of DNA repair pathways in mammalian cells. Complementation studies using these cells have illustrated the genetic and biochemical complexity of these pathways3,5,6. The precise nature of the genes and gene products involved in these mutants has not yet been resolved. Isolation of repair genes by recombinant DNA technology would open up new approaches to the elucidation of repair mechanisms in mammalian cells. Here we report the molecular cloning of a human repair gene (ERCC1) that complements the repair defect in a Chinese hamster ovary (CHO) mutant cell line.Keywords
This publication has 21 references indexed in Scilit:
- Molecular identification of a human DNA repair gene following DNA-mediated gene transferNature, 1983
- Repair capability and the cellular age response for killing and mutation induction after UVMutation Research - Reviews in Mutation Research, 1982
- Isolation of UV-sensitive mutants of mouse L5178Y cells by a cell suspension spotting methodSomatic Cell and Molecular Genetics, 1982
- Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells.Proceedings of the National Academy of Sciences of the United States of America, 1981
- Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.Proceedings of the National Academy of Sciences of the United States of America, 1981
- DNA RepairInternational Review of Cytology, 1981
- Isolation of transforming DNA: Cloning the hamster aprt geneCell, 1980
- Altering Genotype and Phenotype by DNA-Mediated Gene TransferScience, 1980
- Transformation of mammalian cells with genes from procaryotes and eucaryotesCell, 1979
- Genetic Heterogeneity of Xeroderma Pigmentosum demonstrated by Somatic Cell HybridizationNature New Biology, 1972