Activated status of basophils in chronic urticaria leads to interleukin-3 hyper-responsiveness and enhancement of histamine release induced by anti-IgE stimulus
- 1 May 2008
- journal article
- Published by Oxford University Press (OUP) in British Journal of Dermatology
- Vol. 158 (5), 979-986
- https://doi.org/10.1111/j.1365-2133.2008.08499.x
Abstract
Background Basophils and mast cells are the main target cells in chronic idiopathic urticaria (CIU). Besides the basopenia, intrinsic defects of the anti-IgE cross-linking signalling pathway of basophils have been described in CIU. Objectives We sought to investigate the profile of expression of activation markers on basophils of patients with CIU and to explore the effect of interleukin (IL)-3 priming upon anti-IgE cross-linking stimuli through expression of activation markers and basophil histamine releasability. Methods Evaluation of the surface expression of Fc epsilon RI alpha, CD63, CD203c and CD123 on whole blood basophils of patients with CIU undergoing autologous serum skin test (ASST) was performed by flow cytometry. The effect of pretreatment with IL-3 in the anti-IgE response was analysed by the expression of basophil activation markers and histamine release using enzyme-linked immunosorbent assay. Results Blood basophils of patients with CIU were reduced in number and displayed increased surface expression of Fc epsilon RI alpha, which was positively correlated with the IgE serum levels. Upregulation of expression of both surface markers CD203c and CD63 was verified on basophils of patients with CIU, regardless of ASST response. High expression of IL-3 receptor on basophils was detected only in ASST+ patients with CIU. Pretreatment with IL-3 upregulated CD203c expression concomitantly with the excreting function of blood basophils and induced a quick hyper-responsiveness to anti-IgE cross-linking on basophils of patients with CIU compared with healthy controls. Conclusions Basophils of patients with CIU showed an activated profile, possibly due to an in vivo priming. Functionally, basophils have high responsiveness to IL-3 stimulation, thereby suggesting that defects in the signal transduction pathway after IgE cross-linking stimuli are recoverable in subjects with chronic urticariaKeywords
This publication has 34 references indexed in Scilit:
- Flow‐assisted allergy diagnosis: current applications and future perspectivesAllergy, 2006
- Increased responsiveness of basophils of patients with chronic urticaria to sera but hypo‐responsiveness to other stimuliClinical and Experimental Allergy, 2005
- Basophil CD63 expression assay on highly sensitized atopic donor leucocytes-a useful method in chronic autoimmune urticariaBritish Journal of Dermatology, 2004
- The Basophil-Specific Ectoenzyme E-NPP3 (CD203c) as a Marker for Cell Activation and Allergy DiagnosisInternational Archives of Allergy and Immunology, 2004
- Chronic idiopathic urticariaCurrent Opinion in Allergy and Clinical Immunology, 2003
- Marked improvement of the basophil activation test by detecting CD203c instead of CD63Clinical and Experimental Allergy, 2003
- Interleukin‐3, but not granulocyte–macrophage colony‐stimulating factor and interleukin‐5, inhibits apoptosis of human basophils through phosphatidylinositol 3‐kinase: requirement of NF‐κB‐dependent and ‐independent pathwaysImmunology, 2002
- Individual Hymenoptera Venom Compounds Induce Upregulation of the Basophil Activation Marker Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 (CD203c) in Sensitized PatientsInternational Archives of Allergy and Immunology, 2002
- Flow cytometric analysis of basophil numbers in chronic urticaria: basopenia is related to serum histamine releasing activityClinical and Experimental Allergy, 1997
- Levels of tryptase, chymase, and FcϵRIα messenger RNA in human skin are unchanged after IgE-dependent stimulation of cutaneous mast cells in vivoJournal of Allergy and Clinical Immunology, 1997