Transfer of diabetes in mice prevented by blockade of adhesion-promoting receptor on macrophages
- 1 December 1990
- journal article
- letter
- Published by Springer Science and Business Media LLC in Nature
- Vol. 348 (6302), 639-642
- https://doi.org/10.1038/348639a0
Abstract
INSULIN-dependent diabetes mellitus (IDDM) is a disease with an autoimmune aetiology. The non-obese diabetic mouse is a good spontaneous animal model of the human disease, with IDDM developing in 50–80% of female mice by the age of 6 months1,2. The disease can be transferred by splenic T cells from diabetic donors and is prevented by T-cell depletion3,4. The mechanism(s) by which the β cell is specifically destroyed is not known, but T cells and macrophages have both been implicated, based on the presence of macrophages in the infiltrated islet and the ability of chronic silica treatment to prevent disease5,6. The monoclonal antibody 5C6 is specific for the myelomonocytic adhesion-promoting type-3 complement receptor (CR3 or CD11b/CD18)7 and does not bind to T cells. Here we show that blockade of macrophage CR3 in vivo prevents intra-islet infiltration by both macrophages and T cells and inhibits development of IDDM. We conclude that both T cells and macrophages have an essential role in the onset of IDDM.Keywords
This publication has 17 references indexed in Scilit:
- The involvement of Ly 2+ T cells in beta cell destructionJournal of Autoimmunity, 1990
- Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytesCell, 1989
- Exacerbation of murine listeriosis by a monoclonal antibody specific for the type 3 complement receptor of myelomonocytic cells. Absence of monocytes at infective foci allows Listeria to multiply in nonphagocytic cells.The Journal of Experimental Medicine, 1989
- Antibody to the murine type 3 complement receptor inhibits T lymphocyte-dependent recruitment of myelomonocytic cells in vivo.The Journal of Experimental Medicine, 1989
- Diabetes in transgenic mice resulting from over-expression of class I histocompatibility molecules in pancreatic β cellsNature, 1988
- Immunotherapy of the Nonobese Diabetic Mouse: Treatment with an Antibody to T-Helper LymphocytesScience, 1988
- Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo.The Journal of Experimental Medicine, 1987
- Tolerance to rat monoclonal antibodies. Implications for serotherapy.The Journal of Experimental Medicine, 1986
- Non-obese-diabetic mice: immune mechanisms of pancreatic ?-cell destructionDiabetologia, 1984
- F4/80, a monoclonal antibody directed specifically against the mouse macrophageEuropean Journal of Immunology, 1981